Linkage Mapping of the Human CSF2 and IL3 Genes

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 88; no. 11; pp. 4821 - 4824
• Main Authors: Frolova, Elena I., Dolganov, Gregory M., Mazo, Ilya A., Smirnov, Dmitzy V., Copeland, Paul, Stewart, Claudia, O'Brien, Stephen J., Dean, Michael
• Format: Journal Article
• Language: English
• Published: Washington, DC National Academy of Sciences of the United States of America 01.06.1991; National Acad Sciences
• Subjects: 550201 - Biochemistry- Tracer Techniques; Alleles; Base Sequence; BASIC BIOLOGICAL SCIENCES; Biological and medical sciences; CARCINOGENESIS; Cell growth; chromosome 5; Chromosome Mapping; CHROMOSOMES; Chromosomes, Human, Pair 5; Classical genetics, quantitative genetics, hybrids; CLONING; DNA; DNA HYBRIDIZATION; DNA-CLONING; Female; Fundamental and applied biological sciences. Psychology; GENE RECOMBINATION; GENES; Genetic Linkage; Genetic loci; GENETIC MAPPING; Genetics of eukaryotes. Biological and molecular evolution; Genomic Library; Genomics; granulocyte-macrophage colony-stimulating factor; Granulocyte-Macrophage Colony-Stimulating Factor - genetics; GROWTH FACTORS; Human; HUMAN CHROMOSOME 5; HUMAN CHROMOSOMES; Humans; HYBRIDIZATION; Interleukin-3 - genetics; Lymphocytes - physiology; LYMPHOKINES; Male; MAPPING; MITOGENS; Molecular Sequence Data; Myeloid leukemia; NUCLEIC ACIDS; Oligonucleotide Probes; OLIGONUCLEOTIDES; ORGANIC COMPOUNDS; PATHOGENESIS; Polymorphism, Restriction Fragment Length; PROTEINS; Restriction fragment length polymorphism; Restriction Mapping; RFLPS; Genetic mapping; Human; Linkage; Restriction fragment length polymorphism; Interleukin 3; B5-Chromosome; Cytokine; Restriction map; Southern blotting; Granulocyte macrophage colony stimulating factor
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.88.11.4821

Interleukin 3 (encoded by the IL3 gene) and granulocyte-macrophage colony-stimulating factor (encoded by the CSF2 gene) are small secreted polypeptides that bind to specific cell surface receptors and regulate the growth, gene expression, and differentiation of many of the hematopoietic cell lineages, particularly nonlymphoid cells. The IL3 and CSF2 genes have been cloned and mapped to human chromosome bands 5q23-31. Only 10 kilobases of DNA separates the two genes, suggesting that they have a common origin and/or regulation. We have cloned 70 kilobases of genomic DNA that includes the IL3 and CSF2 genes, as well as flanking sequences, and report a physical map of this region. Several unique-sequence DNA segments have been identified in this region, and one of these fragments detects two restriction fragment length polymorphisms in DNA from unrelated Caucasians. Segregation of these DNA polymorphisms was followed in the Centre Etude du Polymorphisme Humaine (CEPH) panel of 40 large three-generation pedigrees, and linkage was detected with 17 genetic markers previously typed in these families. Multipoint linkage analysis permits the placement of the region containing the IL3 and CSF2 structural genes on the recombination-genetic linkage map of chromosome 5q and thereby allows the role of these genes in leukemogenesis to be more critically examined.