Relation between dietary acrylamide exposure and biomarkers of internal dose in Canadian teenagers

• Published in: Journal of exposure science & environmental epidemiology Vol. 24; no. 2; pp. 215 - 221
• Main Authors: Brisson, Benjamin, Ayotte, Pierre, Normandin, Louise, Gaudreau, Éric, Bienvenu, Jean- François, Fennell, Timothy R, Blanchet, Carole, Phaneuf, Denise, Lapointe, Caroline, Bonvalot, Yvette, Gagné, Michelle, Courteau, Marilène, Snyder, Rodney W, Bouchard, Michèle
• Format: Journal Article
• Language: English
• Published: New York Nature Publishing Group US 01.03.2014; Nature Publishing Group
• Subjects: 692/420; 692/53; 692/700/478/174; Acrylamide; Acrylamide - toxicity; Adducts; Adolescent; Adolescents; Adult; Biological markers; Biomarkers; Biomarkers - metabolism; Biomonitoring; Blood; Canada; Carcinogens; Creatinine; Diet; Dietary intake; Dose-Response Relationship, Drug; Environmental Exposure; Epidemiology; Exposure; Female; Food; Food and nutrition; Health aspects; Health risks; Hemoglobin; Humans; Medicine; Medicine & Public Health; Metabolites; Middle Aged; original-article; Passive smoking; Physiological aspects; Subgroups; Teenagers; Urine; Valine; Youth; Canada; Canada, Quebec, Montreal; Canadian teenagers; hemoglobin adducts; LC-MS/MS; acrylamide; biomarkers of exposure; urinary metabolites
• ISSN: 1559-0631; 1559-064X
• DOI: 10.1038/jes.2013.34

Acrylamide (AA) is a probable human carcinogen found in several foods. Little information is available regarding exposure of adolescents, a subgroup potentially consuming more AA-rich foods. We investigated the relationship between dietary AA intake and levels of biomarkers of exposure (urinary metabolites and hemoglobin adducts) in 195 non-smoking teenagers of Montreal Island aged 10–17 years. Dietary habits and personal characteristics were documented by questionnaire. AA and its metabolites were quantified in 12-h urine collections by LC-MS/MS. Hemoglobin adducts from 165 blood samples were also analyzed by LC-MS/MS. Most prevalent urinary metabolites were NACP and NACP-S, with respective geometric mean concentrations of 31.2 and 14.2  μ mol/mol creatinine. Geometric mean concentrations of AAVal and GAVal (hemoglobin adducts of AA and glycidamide (GA) with N-terminal valine residues) were 45.4 and 45.6 pmol/g globin, respectively. AA intake during the 2 days before urine collection was a significant predictor of NACP+NACP-S urinary concentrations ( P <0.0001). AA intakes during the month before blood collection ( P <0.0001) and passive smoking ( P <0.05) were associated with adduct levels. Levels of hemoglobin adducts were above biomonitoring equivalent values corresponding to a 1 × 10 −4 excess cancer risk, which may indicate the need to reduce AA exposure in the population.