Extraadrenal Immunohistochemical Distribution of Steroid 21-Hydroxylase in Human

• Published in: The Tohoku Journal of Experimental Medicine Vol. 154; no. 1; pp. 21 - 28
• Main Authors: SASANO, HIRONOBU, SASANO, NOBUAKI
• Format: Journal Article
• Language: English
• Published: Japan Tohoku University Medical Press 1988
• Subjects: Breast - enzymology; Colon - enzymology; extraadrenal tissues; Female; Humans; Immunohistochemistry; Kidney - enzymology; Liver - enzymology; mineralocorticoid action; P-450C21; Pancreas - enzymology; Salivary Glands - enzymology; Steroid 21-Hydroxylase - analysis; Steroid Hydroxylases - analysis
• ISSN: 0040-8727; 1349-3329
• DOI: 10.1620/tjem.154.21

SASANO, H. and SASANO, N. Extraadrenal Immunohistochemical Distribution of Steroid 21-Hydroxylase in Human. Tohoku J. exp. Med., 1988, 154 (1), 21-28 - Using an antibody against cytochrome P-450 specific for steroid 21- hydroxylase (P-450C21), extraadrenal distribution and localization of the enzyme were immunohistochemically examined on various normal human tissues. Immunoreactive P-450C21 was demonstrated in the renal tubular system, especially in distal tubules and cortical and medullary collecting tubules; excretory ducts including intercalated ducts of the pancreas and salivary glands; secretory ducts of the mammary glands. The immunoreactivity for P-450C21 was not present in the colon and liver. Transepithelial transport of sodium, potassium and hydrogen ions occurs in those sites where P-450C21 is present and mineralocorticoid receptors are presumed to be present. Relationship of extraadrenal 21-hydroxylase, which produces deoxycorticosterone locally, to a mineralocorticoid action is proposed.