Colostral antibody-mediated and cell-mediated immunity contributes to innate and antigen-specific immunity in piglets

• Published in: Developmental and comparative immunology Vol. 43; no. 1; pp. 114 - 120
• Main Authors: Bandrick, Meggan, Ariza-Nieto, Claudia, Baidoo, Samuel K., Molitor, Thomas W.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Ltd 01.03.2014
• Subjects: Adaptive Immunity; Animals; Animals, Newborn; Animals, Suckling; Antigens - immunology; CD4-Positive T-Lymphocytes - immunology; CD8-Positive T-Lymphocytes; Cell Proliferation; Cells, Cultured; Colostrum; Colostrum - immunology; Female; Immunity, Cellular; Immunity, Humoral; Immunity, Innate; Immunity, Maternally-Acquired; Immunoglobulins - metabolism; Killer Cells, Natural - immunology; Maternally derived immunity; Neonate; Passive transfer; Pregnancy; Receptors, Antigen, T-Cell, gamma-delta - metabolism; Swine; Swine - immunology; T lymphocyte; S:P ratio; IgA; PS; M. hyopneumoniae; IgG; CFSE; PBMC; conA; AS; Neonate; 7AAD; APC; Maternally derived immunity; Swine; CMC; Passive transfer; Colostrum; T lymphocyte; AMI; NK; CMI; ELISA; concanavalin A; sample to positive ratio; after-suckling; natural killer; antibody-mediated immunity; colostral mononuclear cells; pre-suckling; 7-aminoactinomycin D; immunoglobulin G; carboxyfluorescein diacetate succinimidyl ester; immunoglobulin A; Mycoplasma hyopneumoniae; enzyme linked immunosorbant assay; antigen presenting cell; peripheral blood mononuclear cell; cell-mediated immunity
• ISSN: 0145-305X; 1879-0089
• DOI: 10.1016/j.dci.2013.11.005

•T lymphocytes from colostrum may transfer into the piglet circulation.•Colostral immunoglobulin transfer into piglet blood unimpeded.•γδ Lymphocytes are found in colostrum.•Offspring of vaccinated dams have antigen-specific lymphoproliferation 1d of age.•Maternal cells may influence the neonate’s immune response. Immunoglobulins and immune cells are critical components of colostral immunity; however, their transfer to and function in the neonate, especially maternal lymphocytes, is unclear. Cell-mediated and antibody-mediated immunity in sow blood and colostrum and piglet blood before (PS) and after (AS) suckling were assessed to investigate transfer and function of maternal immunity in the piglet. CD4, CD8, and γδ lymphocytes were found in sow blood and colostrum and piglet blood PS and AS; each had a unique T lymphocyte profile. Immunoglobulins were detected in sow blood, colostrum, and in piglet blood AS; the immunoglobulin profile of piglet serum AS mimicked that of sow serum. These results suggest selectivity in lymphocyte concentration into colostrum and subsequent lymphocyte transfer into the neonate, but that immunoglobulin transfer is unimpeded. Assessment of colostral natural killer activity and antigen-specific proliferation revealed that colostral cells are capable of influencing the innate and specific immune response of neonatal pigs.