Tumor Promoters Inhibit Spontaneous Differentiation of Friend Erythroleukemia Cells in Culture
Clones of Friend erythroleukemia cells, characterized by the presence of 40-70% benzidine-positive cells synthesizing hemoglobin in the absence of inducing drugs, were treated with several phorbol diesters with a known range of tumor-promoting activity on mouse skin. Good correlation was found betwe...
Saved in:
Published in | Proceedings of the National Academy of Sciences - PNAS Vol. 74; no. 7; pp. 2894 - 2898 |
---|---|
Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
United States
National Academy of Sciences of the United States of America
01.07.1977
National Acad Sciences |
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | Clones of Friend erythroleukemia cells, characterized by the presence of 40-70% benzidine-positive cells synthesizing hemoglobin in the absence of inducing drugs, were treated with several phorbol diesters with a known range of tumor-promoting activity on mouse skin. Good correlation was found between the reported tumor-promoting activity of a particular phorbol diester and its ability to inhibit spontaneous erythroid differentiation in culture. The inhibition of differentiation by 12-O-tetradecanoyl-phorbol-13-acetate, the most active tumor promoter, was maximum after 4 days of treatment; this inhibition was reversed by removal of the phorbol diester no matter how long the period of treatment. Unlike control cells, which gradually revert to a population with a low percentage of benzidine-positive cells, cells treated with 12-O-tetradecanoyl-phorbol-13-acetate retained a high potential for spontaneous differentiation. |
---|---|
ISSN: | 0027-8424 1091-6490 |
DOI: | 10.1073/pnas.74.7.2894 |