Decreased Bone Mineral Density in Subjects Carrying Familial Defective Apolipoprotein B-100

• Published in: The journal of clinical endocrinology and metabolism Vol. 98; no. 12; pp. E1999 - E2005
• Main Authors: Yerges-Armstrong, Laura M., Shen, Haiqing, Ryan, Kathleen A., Streeten, Elizabeth A., Shuldiner, Alan R., Mitchell, Braxton D.
• Format: Journal Article
• Language: English
• Published: United States Oxford University Press 01.12.2013; Copyright by The Endocrine Society; Endocrine Society
• Subjects: Adult; Aged; Aged, 80 and over; Alleles; Amino Acid Substitution; Amish; Apolipoprotein B; Apolipoprotein B-100 - genetics; Apolipoprotein B-100 - metabolism; Apolipoproteins; Arteriosclerosis; Body mass index; Bone Density; Bone mineral density; Bone Resorption - etiology; Cardiovascular diseases; Cholesterol; Cholesterol, LDL - blood; Cross-Sectional Studies; Dual energy X-ray absorptiometry; Epidemiology; Female; Founder Effect; Genotypes; Heart diseases; Heterozygote; Humans; Hyperlipidemia; Hyperlipoproteinemia Type II - blood; Hyperlipoproteinemia Type II - genetics; Hyperlipoproteinemia Type II - metabolism; Hyperlipoproteinemia Type II - physiopathology; JCEM Online: Advances in Genetics; Low density lipoprotein; Male; Metabolic syndrome; Middle Aged; Mutation; Mutation Rate; Osteoporosis; Pennsylvania; Point Mutation; Population genetics; Population studies; Spine (lumbar); Young Adult; Pennsylvania
• ISSN: 0021-972X; 1945-7197; 1945-7197
• DOI: 10.1210/jc.2013-2471

Context:Although numerous epidemiologic studies have documented associations between osteoporosis and cardiovascular disease, the mechanisms underlying this association remain to be clarified. One hypothesis is that hyperlipidemia may be a common predisposing factor to both atherosclerotic heart disease and bone fragility.Objective:To evaluate this, we compared bone mineral density (BMD) between subjects with and without the R3500Q APOB mutation, the cause of familial defective apolipoprotein B-100, which has been previously shown to markedly increase low-density lipoprotein cholesterol (LDL-C). We hypothesized that R3500Q carriers would have lower BMD due to lifetime, elevated LDL-C.Design:This was a a cross-sectional study in the Old Order Amish (OOA) population.Participants:The R3500Q APOB mutation is present at a high frequency (∼6% vs <0.5%) in the OOA population due to a founder effect. Therefore, we conducted analysis on 1097 Amish individuals of whom 125 were R3500Q carriers.Main Outcome Measure:BMD was measured by dual-energy x-ray absorptiometry.Results:After adjusting for age, age2, sex, body mass index, and family structure, carriers for the Q risk allele had significantly lower BMD than noncarriers at the femoral neck (P = .037), lumbar spine (P = .035) and whole body (P = .016). Adjusting for LDL-C attenuated the association between R3500Q genotype and BMD but did not completely explain the relationship. Subgroup analyses showed no significant interactions with sex, age, or presence of metabolic syndrome.Conclusion:These results use the unique genetic architecture of the OOA population to provide a novel line of evidence supporting a causal role for elevated LDL-C in lowering BMD.