Chemical and Biological Properties of Lipopolysaccharides from Symbiotic Luminous Bacteria from Several Luminous Marine Animals

• Published in: Microbiology and immunology Vol. 27; no. 2; pp. 137 - 149
• Main Authors: Kuwae, Toyoyasu, Kurata, Munetsugu
• Format: Journal Article
• Language: English
• Published: Australia Blackwell Publishing Ltd 01.02.1983
• Subjects: Acropoma japonicum; Adjuvants, Immunologic; Animals; Antibody Formation; Bacteria; Chlorophthalmus; Coelorhynchus; Doryteuthis kensaki; Electrophoresis, Polyacrylamide Gel; Enterobacteriaceae - physiology; Immune Tolerance; Lipopolysaccharides - analysis; Lipopolysaccharides - physiology; Luminescent Measurements; Lymphocyte Activation; Macrophages - immunology; Marine; Mice; Phagocytosis; Photobacterium phosphoreum; Polysaccharides, Bacterial - physiology; Ventrifossa
• ISSN: 0385-5600; 1348-0421
• DOI: 10.1111/j.1348-0421.1983.tb03578.x

The chemical and biological properties of lipopolysaccharides (LPS) in five strains of symbiotic luminous bacteria isolated from four species of luminous marine fishes, Coelorhynchus kishinouyei (CK‐1), Chlorophthalmus albatrossis (CA‐1), Ventrifossa garmani (VG‐1), and Acropoma japonicum (AJ‐1b), as well as from a luminous squid, Doryteuthis kensaki (DK‐1) were examined. The LPS isolated from these symbiotic luminous bacteria were characterized by the absence of 2‐keto‐3‐deoxyoctonate, known to be a basic component of the usual gram‐negative bacterial LPS. All LPS from these symbiotic luminous bacteria upon electrophoresis in sodium dodecylsulfate polyacrylamide gel exhibited one or two clear main bands with high mobility, and one or two obscure minor bands with low mobility when stained with periodate‐Schiff reagent. LPS from CA‐1 and VG‐1 exhibited similar electrophoretic patterns, whereas the electrophoretic patterns of the LPS from CK‐1, AJ‐1b, and DK‐1 were easily distinguishable from each other. All these LPS also had similarly potent and diverse biological activities in regard to their adjuvanticity, immunosuppression, polyclonal effect, B‐cell mitogenicity, and activation of the phagocytic function of macrophages.