A Monoclonal Antibody Defining Human B Cell Differentiation Antigen (HLB-1 Antigen)

• Published in: Microbiology and immunology Vol. 27; no. 1; pp. 51 - 64
• Main Authors: Kasai, Kiyoshi, Koshiba, Hirofumi, Ishii, Yoshifumi, Kikuchi, Koukichi
• Format: Journal Article
• Language: English
• Published: Australia Blackwell Publishing Ltd 01.01.1983
• Subjects: Animals; Antibodies, Monoclonal - immunology; Antigens, Differentiation, B-Lymphocyte; Antigens, Surface - immunology; B-Lymphocytes - cytology; B-Lymphocytes - immunology; Bone Marrow - immunology; Cell Differentiation; Cell Line; Epstein-Barr virus; Humans; Hybridomas - immunology; Leukemia - immunology; Lymphoid Tissue - immunology; Lymphoma - immunology; Mice; Mice, Inbred BALB C; Multiple Myeloma - immunology
• ISSN: 0385-5600; 1348-0421
• DOI: 10.1111/j.1348-0421.1983.tb03567.x

A new monoclonal antibody specific for human B cell differentiation antigen (HLB‐1) is produced by a hybridoma established by fusion of splenocytes of mice immunized with the Epstein‐Barr virus (EBV)‐transformed peripheral B cell line, RPMI‐8057. This monoclonal, antibody designated anti‐HLB‐1 monoclonal antibody (anti‐HLB‐1), reacted with surface immunoglobulin (sIg)‐positive B cells of normal peripheral blood and lymphoid tissues and sIg‐positive leukemic cells. The cells of T cell leukemia, non‐T non‐B acute lymphoblastic leukemia (ALL) and nonlymphoid leukemia were HLB‐1 negative. These data were further confirmed by studying a panel of cultured human hematopoietic cell lines. Anti‐HLB‐1 reacted with B cell lines derived from pre‐B, Burkitt's lymphoma, B cell type ALL and EBV‐transformed peripheral B cells. Anti‐HLB‐1 was reactive with only one of three human myeloma cell lines, and with none of the T cell, myeloid and non‐T non‐B ALL cell lines. This newly defined HLB‐1 antigen is different from other conventional human B cell markers such as sIg, Ia antigens, and receptors for the Fc portion of Ig and complement C3.