Cotransplantation of mesenchymal stem cells and endothelial progenitor cells for treating steroid‐induced osteonecrosis of the femoral head

• Published in: Stem cells translational medicine Vol. 10; no. 5; pp. 781 - 796
• Main Authors: Xu, Haixia, Wang, Chengqiang, Liu, Chun, Peng, Ziyue, Li, Jianjun, Jin, Yanglei, Wang, Yihan, Guo, Jiasong, Zhu, Lixin
• Format: Journal Article
• Language: English
• Published: Hoboken, USA John Wiley & Sons, Inc 01.05.2021; Oxford University Press
• Subjects: Adipogenesis; Alginic acid; Angiogenesis; Animals; Antibodies; Biocompatibility; Bone marrow; bone marrow mesenchymal stem cells; Bone surgery; Bones; Cell adhesion; Cell Differentiation; Chitosan; Comparative analysis; Cytotoxicity; Decompression; Disease; endothelial progenitor cells; Endothelial Progenitor Cells - transplantation; Endothelium; Femur; Femur Head; Femur Head Necrosis - chemically induced; Femur Head Necrosis - therapy; Freeze drying; Growth factors; Health aspects; Medical research; Mesenchymal Stem Cell Transplantation; Mesenchymal stem cells; Necrosis; Osteogenesis; Osteonecrosis; osteonecrosis of the femoral head; Porosity; Progenitor cells; Rabbits; Stem cells; Steroids - adverse effects; Tissue engineering; Tissue Engineering and Regenerative Medicine; Tissue Scaffolds; Transplants & implants; China; Germany; United States > US; adipogenesis; osteogenesis; bone marrow mesenchymal stem cells; angiogenesis; endothelial progenitor cells; osteonecrosis of the femoral head
• ISSN: 2157-6564; 2157-6580; 2157-6580
• DOI: 10.1002/sctm.20-0346

Steroid‐induced osteonecrosis of the femoral head (ONFH) is characterized by decreased osteogenesis, angiogenesis, and increased adipogenesis. While bone tissue engineering has been widely investigated to treat ONFH, its therapeutic effects remain unsatisfactory. Therefore, further studies are required to determine optimal osteogenesis, angiogenesis and adipogenesis in the necrotic area of the femoral head. In our study, we developed a carboxymethyl chitosan/alginate/bone marrow mesenchymal stem cell/endothelial progenitor cell (CMC/ALG/BMSC/EPC) composite implant, and evaluated its ability to repair steroid‐induced ONFH. Our in vitro studies showed that BMSC and EPC coculture displayed enhanced osteogenic and angiogenic differentiation. When compared with single BMSC cultures, adipogenic differentiation in coculture systems was reduced. We also fabricated a three‐dimensional (3D) CMC/ALG scaffold for loading cells, using a lyophilization approach, and confirmed its good cell compatibility characteristics, that is, high porosity, low cytotoxicity and favorable cell adhesion. 3D coculture of BMSCs and EPCs also promoted secretion of osteogenic and angiogenic factors. Then, we established an rabbit model of steroid‐induced ONFH. The CMC/ALG/BMSC/EPC composite implant was transplanted into the bone tunnel of the rabbit femoral head after core decompression (CD) surgery. Twelve weeks later, radiographical and histological analyses revealed CMC/ALG/BMSC/EPC composite implants had facilitated the repair of steroid‐induced ONFH, by promoting osteogenesis and angiogenesis, and reducing adipogenesis when compared with CD, CMC/ALG, CMC/ALG/BMSC and CMC/ALG/EPC groups. Thus, our data show that cotransplantation of BMSCs and EPCs in 3D scaffolds is beneficial in treating steroid‐induced ONFH. Bone marrow mesenchymal stem cell (BMSCs) and endothelial progenitor cell (EPCs) isolated from the tibia medullary cavity of rabbits were seeded in the carboxymethyl chitosan (CMC)/alginate (ALG) scaffold to construct the CMC/ALG/BMSCs/EPCs composite implant. Then, the composite implant was transplanted into the necrotic femoral head induced by lipopolysaccharide (LPS) and methylprednisolone (MPS). Repair effects of BMSCs/EPCs on steroid‐induced osteonecrosis of the femoral head (ONFH) were confirmed by increased osteogenesis and angiogenesis and decreased adipogenesis.