Community Structure of Endophytic Bacteria in Chestnut, Castanea mollissima

[ Objective] The paper was to detect the species of endophytic bacteria in Chestnut （Castanea mollissima）. [ Method ] The 16S rDNA-V4 region of endophytic bacteria in chestnut （sample name BL） was sequenced by Illumina MiSeq high-throughput sequencing technology. The number of sequences and op- erat...

Full description

Saved in:
Bibliographic Details
Published inPlant Diseases and Pests(植物病虫害研究：英文版) Vol. 7; no. 4; pp. 1 - 5
Main Authors Zebin, Chen, Ring, Li, Tiyuan, Xia, Dingkang, Wang, Lei, Yu, Shengguang, Xu, Jiani, Liu, Yongfu, Zhang
Format Journal Article
LanguageEnglish
Published Cranston Wu Chu (USA-China) Science and Culture Media Corporation 01.08.2016
Online AccessGet full text

Cover

More Information
Summary:[ Objective] The paper was to detect the species of endophytic bacteria in Chestnut （Castanea mollissima）. [ Method ] The 16S rDNA-V4 region of endophytic bacteria in chestnut （sample name BL） was sequenced by Illumina MiSeq high-throughput sequencing technology. The number of sequences and op- erational taxonomic units （OTUs） for each sample was sorted and calculated using Qiime and Mothur software. The abundance and α-diversity of species were analyzed. [ Result] The number of effective sequences and OTUs were 21 167/191. The rarefaction curves showed that adequate sampling was achieved, and the number of OTUs was close to saturation. The endophytic bacteria of chestnut belonged to 10 genera, including Koribacter （3.48%） , Solibacter （2.59%） , Bra- d~hizobium （ 3. 57% ）, Rhodoplanes （ 7. 41% ）, Methylobacterium （ 4. 20% ）, Agrobacterium （ 19. 73% ）, Kaistobacter （ 2. 05% ）, Sphingomonas （18.66%）, Acidovorax （35.98%）, and Methylibium （2.32%）. The dominant species were Acidovorax, Agrobacterium and Sphingomonas. [ Conclusion] Illumina high-throughput sequencing technology provided more accurate and scientific data resources for the study of endophytic bacteria in chestnut.
Bibliography:[ Objective] The paper was to detect the species of endophytic bacteria in Chestnut （Castanea mollissima）. [ Method ] The 16S rDNA-V4 region of endophytic bacteria in chestnut （sample name BL） was sequenced by Illumina MiSeq high-throughput sequencing technology. The number of sequences and op- erational taxonomic units （OTUs） for each sample was sorted and calculated using Qiime and Mothur software. The abundance and α-diversity of species were analyzed. [ Result] The number of effective sequences and OTUs were 21 167/191. The rarefaction curves showed that adequate sampling was achieved, and the number of OTUs was close to saturation. The endophytic bacteria of chestnut belonged to 10 genera, including Koribacter （3.48%） , Solibacter （2.59%） , Bra- d~hizobium （ 3. 57% ）, Rhodoplanes （ 7. 41% ）, Methylobacterium （ 4. 20% ）, Agrobacterium （ 19. 73% ）, Kaistobacter （ 2. 05% ）, Sphingomonas （18.66%）, Acidovorax （35.98%）, and Methylibium （2.32%）. The dominant species were Acidovorax, Agrobacterium and Sphingomonas. [ Conclusion] Illumina high-throughput sequencing technology provided more accurate and scientific data resources for the study of endophytic bacteria in chestnut.
High-throughput sequencing; Detection; Chestnut; Endophytic bacteria
SourceType-Scholarly Journals-1
ObjectType-General Information-1
content type line 14
ISSN:2152-3932