Heterogeneity of engrafted bone-lining cells after systemic and local transplantation

• Published in: Blood Vol. 106; no. 10; pp. 3650 - 3657
• Main Authors: Wang, Liping, Liu, Yaling, Kalajzic, Zana, Jiang, Xi, Rowe, David W.
• Format: Journal Article
• Language: English
• Published: Washington, DC Elsevier Inc 15.11.2005; The Americain Society of Hematology; 2005 by The American Society of Hematology
• Subjects: Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy; Animals; Biological and medical sciences; Bone Marrow Transplantation - methods; Bone marrow, stem cells transplantation. Graft versus host reaction; Calcification, Physiologic - physiology; Extracellular Matrix - metabolism; Gene Expression Regulation - physiology; Humans; Medical sciences; Mice; Mice, Transgenic; Models, Animal; Organ Specificity - physiology; Organ Specificity - radiation effects; Osteoblasts - cytology; Osteoblasts - physiology; Osteogenesis - physiology; Osteogenesis - radiation effects; Skull - cytology; Skull - physiology; Stromal Cells - cytology; Stromal Cells - physiology; Transfusions. Complications. Transfusion reactions. Cell and gene therapy; Transplantation; Transplantation Chimera - physiology; Transplantation, Homologous; Whole-Body Irradiation - methods; Animal model; Stem cell; Rodentia; Homograft; Cell differentiation; Osteoarticular system; Vertebrata; Calvaria; Mammalia; Mouse; Surgery; Animal; Bone marrow; Osteoblast; Graft; Stromal cell; Bone; Osteogenesis
• ISSN: 0006-4971; 1528-0020
• DOI: 10.1182/blood-2005-02-0582

The outcome of various osteoprogenitor-cell transplantation protocols was assessed using Col1a1-GFP reporter transgenic mice. The model requires the recipient mice to undergo lethal total body irradiation (TBI) followed by rescue with whole bone marrow. When the mice are rescued with total bone marrow from a Col1a1-GFP transgenic mouse, green fluorescence protein (GFP)-positive donor cells can be observed on most endosteal and trabecular bone surfaces. Although the cells express an osteoblast-restricted GFP, they fail to progress to osteocytes, do not form a mineralized matrix, and do not generate bone nodules in vitro. However when calvarial progenitor cells derived from the same transgenic mice are injected into the bone marrow space, osteogenesis by the donor cells is observed. Using different GFP colors that distinguish the donor and recipient osteoblasts, commingling of the 2 cells types is observed along the mineralizing osteoblast surface as well as within the osteocyte population of the endosteal bone. Despite the ability of the injected progenitor cells to produce bone within the injected bone, they lack the ability to form mineralized bone nodules when explanted to primary osteoblast culture. These reagents and imaging protocols will be useful in evaluating other cells having a better progenitor potential than calvarial-derived stromal cells.