Colloidal Nanoparticles Isolated from Duck Soup Exhibit Antioxidant Effect on Macrophages and Enterocytes

• Published in: Foods Vol. 12; no. 5; p. 981
• Main Authors: Xu, Ligen, Duan, Mingcai, Cai, Zhaoxia, Zeng, Tao, Sun, Yangying, Cheng, Shuang, Xia, Qiang, Zhou, Changyu, He, Jun, Lu, Lizhi, Pan, Daodong
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 25.02.2023; MDPI
• Subjects: antioxidant effect; Antioxidants; Apoptosis; Aquatic birds; Caco-2; Carbohydrates; Cell lines; Chemical properties; colloidal nanoparticles; Colloiding; Cooking; duck soup; Enterocytes; Enzymes; Food; Food processing; Food science; Free radicals; Health aspects; Homeostasis; Identification and classification; Intestine; Lipids; Macrophages; Nanoparticles; Nutritional aspects; Oxidative stress; Potassium; Proteins; RAW 264.7; Reagents; Scavenging; Soups; Waterfowl; China; United States > US; colloidal nanoparticles; RAW 264.7; duck soup; Caco-2; antioxidant effect
• ISSN: 2304-8158; 2304-8158
• DOI: 10.3390/foods12050981

Food-derived colloidal nanoparticles (CNPs) have been found in many food cooking processes, and their specific effects on human health need to be further explored. Here, we report on the successful isolation of CNPs from duck soup. The hydrodynamic diameters of the obtained CNPs were 255.23 ± 12.77 nm, which comprised lipids (51.2%), protein (30.8%), and carbohydrates (7.9%). As indicated by the tests of free radical scavenging and ferric reducing capacities, the CNPs possessed remarkable antioxidant activity. Macrophages and enterocytes are essential for intestinal homeostasis. Therefore, RAW 264.7 and Caco-2 were applied to establish an oxidative stress model to investigate the antioxidant characteristics of the CNPs. The results showed that the CNPs from duck soup could be engulfed by these two cell lines, and could significantly alleviate 2,2'-Azobis(2-methylpropionamidine) dihydrochloride (AAPH)-induced oxidative damage. It indicates that the intake of duck soup is beneficial for intestinal health. These data contribute to revealing the underlying functional mechanism of Chinese traditional duck soup and the development of food-derived functional components.