Fermentation characteristics and microbial community composition of wet brewer's grains and corn stover mixed silage prepared with cellulase and lactic acid bacteria supplementation

• Published in: Animal Bioscience Vol. 37; no. 1; pp. 84 - 94
• Main Authors: Zhao, Guoqiang, Wu, Hao, Li, Yangyuan, Li, Li, He, Jiajun, Yang, Xinjian, Xie, Xiangxue
• Format: Journal Article
• Language: English
• Published: Korea (South) Asian - Australasian Association of Animal Production Societies 01.01.2024; Animal Bioscience; Asian-Australasian Association of Animal Production Societies; 아세아·태평양축산학회
• Subjects: Bacteria; Breweries; Brewing industry; cellulase; Contamination; Corn; corn stover; Distillers feeds; Enzymes; Fermentation; fermentation quality; Health aspects; Instrument industry; lactic acid bacteria; Microbial colonies; microbial community; Production processes; Silage; wet brewer’s grains; 축산학; United States; China; Wet Brewer’s Grains; Corn Stover; Fermentation Quality; Lactic Acid Bacteria; Microbial Community; Cellulase
• ISSN: 2765-0189; 2765-0235
• DOI: 10.5713/ab.23.0177

The objective of this study was to investigate how cellulase or/and lactic acid bacteria (LAB) affected the fermentation characteristic and microbial community in wet brewer's grains (WBG) and corn stover (CS) mixed silage. The WBG was mixed thoroughly with the CS at 7:3 (w/w). Four treatment groups were studied: i) CON, no additives; ii) CEL, added cellulase (120 U/g fresh matter [FM]), iii) LAB, added LAB (2×106 cfu/g FM), and iv) CLA, added cellulase (120 U/g FM) and LAB (2×106 cfu/g FM). All additive-treated groups showed higher fermentation quality over the 30 d ensiling period. As these groups exhibited higher (p<0.05) LAB counts and lactic acid (LA) content, along with lower pH value and ammonia-nitrogen (NH3-N) content than the control. Specifically, cellulase-treated groups (CEL and CLA) showed lower (p<0.05) neutral detergent fiber and acid detergent fiber contents than other groups. All additives increased the abundance of beneficial bacteria (Firmicutes, Lactiplantibacillus, and Limosilactobacillus) while they decreased abundance of Proteobacteria and microbial diversity as well. The combined application of cellulase and LAB could effectively improve the fermentation quality and microbial community of the WBG and CS mixed silage.