Multi-omics analysis revealed that the protein kinase MoKin1 affected the cellular response to endoplasmic reticulum stress in the rice blast fungus, Magnaporthe oryzae

• Published in: BMC genomics Vol. 25; no. 1; pp. 449 - 15
• Main Authors: Zhang, Lianhu, Zhang, Yifan, Liu, Yankun, Miao, Wenjing, Ai, Jingyu, Li, Jingling, Peng, Song, Li, Songyan, Ye, Lifang, Zeng, Rong, Shi, Xugen, Ma, Jian, Lin, Yachun, Kuang, Weigang, Cui, Ruqiang
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 07.05.2024; BioMed Central; BMC
• Subjects: Agricultural production; Amino acids; Analysis; Ascomycota; Biochemistry; Carbohydrates; Cell cycle; Defects; Disease; Down-regulation; Endoplasmic reticulum; Endoplasmic Reticulum Stress; ER stress; Explosions; Fungal Proteins - genetics; Fungal Proteins - metabolism; Fungi; Gene Expression Regulation, Fungal; Genes; Genetic transcription; Genetic translation; Germination; Glycerol; Homeostasis; Infections; Kinases; Leaves; Magnaporthe oryzae; Metabolic disorders; Mokin1; Multiomics; Mutants; Mutation; Oryza - genetics; Oryza - microbiology; Pathogenicity; Pathogens; Pesticides; Phosphoproteomic; Phosphorylation; Physiological aspects; Physiology; Plant Diseases - microbiology; Protein folding; Protein kinase; Protein kinases; Protein Kinases - genetics; Protein Kinases - metabolism; Proteins; Proteomic; Proteomics; Rice; Rice blast; RNA; Signal transduction; Stress; Transcriptomic; Mokin1; ER stress; Phosphoproteomic; Transcriptomic; Proteomic
• ISSN: 1471-2164; 1471-2164
• DOI: 10.1186/s12864-024-10337-8

Previous studies have shown that protein kinase MoKin1 played an important role in the growth, conidiation, germination and pathogenicity in rice blast fungus, Magnaporthe oryzae. ΔMokin1 mutant showed significant phenotypic defects and significantly reduced pathogenicity. However, the internal mechanism of how MoKin1 affected the development of physiology and biochemistry remained unclear in M. oryzae. This study adopted a multi-omics approach to comprehensively analyze MoKin1 function, and the results showed that MoKin1 affected the cellular response to endoplasmic reticulum stress (ER stress). Proteomic analysis revealed that the downregulated proteins in ΔMokin1 mutant were enriched mainly in the response to ER stress triggered by the unfolded protein. Loss of MoKin1 prevented the ER stress signal from reaching the nucleus. Therefore, the phosphorylation of various proteins regulating the transcription of ER stress-related genes and mRNA translation was significantly downregulated. The insensitivity to ER stress led to metabolic disorders, resulting in a significant shortage of carbohydrates and a low energy supply, which also resulted in severe phenotypic defects in ΔMokin1 mutant. Analysis of MoKin1-interacting proteins indicated that MoKin1 really took participate in the response to ER stress. Our results showed the important role of protein kinase MoKin1 in regulating cellular response to ER stress, providing a new research direction to reveal the mechanism of MoKin1 affecting pathogenic formation, and to provide theoretical support for the new biological target sites searching and bio-pesticides developing.