Lipid Polyunsaturated Fatty Acid Chains in Mouse Kidneys Were Increased within 5 min of a Single High Dose Whole Body Irradiation

• Published in: International journal of molecular sciences Vol. 24; no. 15; p. 12439
• Main Authors: Li, Wenxin, Zhang, Chi, Aramaki, Shuhei, Xu, Lili, Tsuge, Shogo, Sakamoto, Takumi, Mamun, Md Al, Islam, Ariful, Hayakawa, Takamitsu, Takanashi, Yusuke, Dubail, Maxime, Konishi, Kenta, Sato, Tomohito, Kahyo, Tomoaki, Fouillade, Charles, Nakamura, Katsumasa, Setou, Mitsutoshi
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 01.08.2023; MDPI
• Subjects: Analysis; Cancer therapies; Fatty acids; high dose radiation; kidney; Kidneys; Lipid peroxidation; lipidomic; Lipids; Liquid chromatography; Liver; Mass spectrometry; polyunsaturated fatty acid; Prostate; Radiation therapy; Scientific equipment and supplies industry; Scientific imaging; Canada; United States; Japan; lipidomic; kidney; high dose radiation; polyunsaturated fatty acid
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms241512439

To understand the ultra-early reaction of normal organ lipids during irradiation, we investigated the response of lipids, including polyunsaturated fatty acid (PUFA) chains, which are particularly susceptible to damage by ROS, in mice's kidneys, lungs, brains, and livers within 5 min of single high-dose irradiation. In this study, we set up three groups of C56BL/6 male mice and conducted whole-body irradiation with 0 Gy, 10 Gy, and 20 Gy single doses. Kidney, lung, brain, and liver tissues were collected within 5 min of irradiation. PUFA-targeted and whole lipidomic analyses were conducted using liquid chromatography-tandem mass spectrometry (LC-MS/MS). The results showed that PUFA chains of kidney phosphatidylcholine (PC), phosphatidylethanolamine (PE), and triacylglycerol (TG) significantly increased within 5 min of 10 Gy and 20 Gy irradiation. The main components of increased PUFA chains in PC and PE were C18:2, C20:4, and C22:6, and in TG the main component was C18:2. The kidney lipidomes also showed significant changes from the perspective of lipid species, mainly dominated by an increase in PC, PE, TG, and signal lipids, while lipidomes of the lung, brain, and liver were slightly changed. Our results revealed that acute PUFA chains increase and other lipidomic changes in the kidney upon whole-body irradiation within 5 min of irradiation. The significantly increased lipids also showed a consistent preference for possessing PUFA chains. The lipidomic changes varied from organ to organ, which indicates that the response upon irradiation within a short time is tissue-specific.