Metabolic Oscillations and Glycolytic Phenotypes of Cancer Cells

Cancer cells show several metabolic phenotypes depending on the cancer types and the microenvironments in tumor tissues. The glycolytic phenotype is one of the hallmarks of cancer cells and is considered to be one of the crucial features of malignant cancers. Here, we show glycolytic oscillations in...

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Bibliographic Details
Published inInternational journal of molecular sciences Vol. 24; no. 15; p. 11914
Main Authors Amemiya, Takashi, Shibata, Kenichi, Yamaguchi, Tomohiko
Format Journal Article
LanguageEnglish
Published Switzerland MDPI AG 25.07.2023
MDPI
Subjects
Cancer
Cancer cells
Cervical cancer
Enzymes
feedback inhibition
Genetic aspects
Glucose
glycolytic oscillations
glycolytic phenotype
Hypothesis
Kinases
malignancy
mathematical model
Mathematical models
Metabolism
Metabolites
Metastasis
Phenotype
Prostate cancer
Variables
Yeast
Japan
mathematical model
cancer cells
glycolytic phenotype
malignancy
feedback inhibition
glycolytic oscillations
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Summary:Cancer cells show several metabolic phenotypes depending on the cancer types and the microenvironments in tumor tissues. The glycolytic phenotype is one of the hallmarks of cancer cells and is considered to be one of the crucial features of malignant cancers. Here, we show glycolytic oscillations in the concentrations of metabolites in the glycolytic pathway in two types of cancer cells, HeLa cervical cancer cells and DU145 prostate cancer cells, and in two types of cellular morphologies, spheroids and monolayers. Autofluorescence from nicotinamide adenine dinucleotide (NADH) in cells was used for monitoring the glycolytic oscillations at the single-cell level. The frequencies of NADH oscillations were different among the cellular types and morphologies, indicating that more glycolytic cancer cells tended to exhibit oscillations with higher frequencies than less glycolytic cells. A mathematical model for glycolytic oscillations in cancer cells reproduced the experimental results quantitatively, confirming that the higher frequencies of oscillations were due to the higher activities of glycolytic enzymes. Thus, glycolytic oscillations are expected as a medical indicator to evaluate the malignancy of cancer cells with glycolytic phenotypes.
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content type line 23
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms241511914