GLP-1 localisation and proglucagon gene expression in healthy and diabetic mouse ileum

• Published in: Journal of veterinary research Vol. 62; no. 2; pp. 237 - 242
• Main Authors: Taşçı, Serap Koral, Bingöl, Seyit Ali
• Format: Journal Article
• Language: English
• Published: Poland Sciendo 01.06.2018; De Gruyter Poland
• Subjects: Avidin; Biotin; Diabetes; Diabetes mellitus; Epithelial cells; Gene expression; GLP-1; Glucagon; Glucagon-like peptide 1; Ileum; Immunohistochemistry; Intestine; L cells; Localization; mice; Peroxidase; Polymerase chain reaction; proglucagon; Reverse transcription; Small intestine; Streptozocin; L cells; diabetes mellitus; GLP-1; proglucagon; mice
• ISSN: 2450-7393; 2450-8608; 2450-8608
• DOI: 10.2478/jvetres-2018-0033

Glucagon-like peptide-1 (GLP-1) is a polypeptide that is mainly produced by intestinal L cells and is encoded by the proglucagon gene. In this study, GLP-1 localisation was investigated in the ileum of healthy and diabetic mice by immunohistochemistry and proglucagon gene expression was assayed by reverse transcription-polymerase chain reaction. This study included 18 male mice that were divided into diabetic, sham, and control groups. Mice in the diabetic group received 100 mg/kg of streptozotocin. Immunohistochemical expression of GLP-1 was determined using the avidin-biotin-peroxidase complex technique, and proglucagon gene expression was determined by RT-PCR. Analysis of GLP-1 immunohistochemical localisation showed that GLP-1-immunopositive cells (L cells) were present between epithelial cells in the intestinal crypts. The intensity and localisation of GLP-1 immunoreactivity were similar among the mice in all the groups. Proglucagon gene expression levels were also statistically similar among the mice in all the groups. No difference was demonstrated among the mice in the diabetic, sham, or control groups with respect to proglucagon gene expression and GLP-1 localisation in the ileum, suggesting that diabetes does not affect proglucagon gene expression in the ileum.