Iron-binding properties of sugar cane yeast peptides

• Published in: Food chemistry Vol. 142; pp. 166 - 169
• Main Authors: de la Hoz, Lucia, Ponezi, Alexandre N., Milani, Raquel F., Nunes da Silva, Vera S., Sonia de Souza, A., Bertoldo-Pacheco, Maria Teresa
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 01.01.2014; Elsevier
• Subjects: Biological and medical sciences; Chemical and industrial products toxicology. Toxic occupational diseases; Dialyzability; Immobilized metal affinity chromatography; Iron - chemistry; Iron-Binding Proteins - chemistry; Iron-Binding Proteins - isolation & purification; Iron-chelating peptides; Medical sciences; Metals and various inorganic compounds; Peptides - chemistry; Peptides - isolation & purification; Protein Binding; Protein hydrolysates; Protein Hydrolysates - chemistry; Saccharomyces cerevisiae; Saccharomyces cerevisiae - chemistry; Saccharomyces cerevisiae Proteins - chemistry; Saccharomyces cerevisiae Proteins - isolation & purification; Subtilisins - chemistry; Sugar-cane yeast; Toxicology; Sugar-cane yeast; Immobilized metal affinity chromatography; Protein hydrolysates; Dialyzability; Iron-chelating peptides; Binding; Yeast; Peptides; Cane sugar; Transition metal; Iron; Protein; Micronutrient; Hydrolysate; Affinity chromatography; Sugar cane
• ISSN: 0308-8146; 1873-7072
• DOI: 10.1016/j.foodchem.2013.06.133

•Fraction of peptides from yeast extract hydrolysates chelates iron immobilized in IMAC at low pH.•Iron binding peptides kept the iron stable during simulated gastro-intestinal digestion in vitro.•Viscozyme hydrolysate from yeast extract showed high iron dializability. The extract of sugar-cane yeast (Saccharomyces cerevisiae) was enzymatically hydrolysed by Alcalase, Protex or Viscozyme. Hydrolysates were fractionated using a membrane ultrafiltration system and peptides smaller than 5kDa were evaluated for iron chelating ability through measurements of iron solubility, binding capacity and dialyzability. Iron-chelating peptides were isolated using immobilized metal affinity chromatography (IMAC). They showed higher content of His, Lys, and Arg than the original hydrolysates. In spite of poor iron solubility, hydrolysates of Viscozyme provided higher iron dialyzability than those of other enzymes. This means that more chelates of iron or complexes were formed and these kept the iron stable during simulated gastro-intestinal digestion in vitro, improving its dialyzability.