High Expression of SLC41A3 Correlates with Poor Prognosis in Hepatocellular Carcinoma

• Published in: OncoTargets and therapy Vol. 14; pp. 2975 - 2988
• Main Authors: Li, Qian, Xiong, Dan-Lei, Wang, Heng, Jin, Wei-Li, Ma, Ying-Yu, Fan, Xiao-Ming
• Format: Journal Article
• Language: English
• Published: New Zealand Dove Medical Press Limited 01.01.2021; Taylor & Francis Ltd; Dove
• Subjects: Analysis; Biomarkers; Cell cycle; Chemotherapy; Development and progression; Gene expression; Gene set enrichment analysis; Genes; Hepatitis; Hepatocellular carcinoma; Hepatoma; Immunohistochemistry; Liver; Liver cancer; Magnesium; Medical prognosis; Metastases; Metastasis; Microvasculature; Original Research; Patients; Prognosis; Proteins; Radiation therapy; RNA; Software; Statistical analysis; Survival analysis; China; United States > US; solute carrier 41A3; HCC; immunohistochemistry; bioinformatics
• ISSN: 1178-6930; 1178-6930
• DOI: 10.2147/OTT.S296187

SLC41A3 is a member of the solute carrier family 41 (SLC41) and is involved in many cellular processes as a magnesium ion transporter. Although it plays an important role in cancer formation and development, the correlation between the expression of SLC41A3 and the occurrence and prognosis of hepatocellular carcinoma (HCC) remains unclear. Therefore, this study was focused on the evaluation of the relationship between SLC41A3 and the development and prognosis of HCC. Firstly, we collected the mRNA expression of SLC41A3 in HCC through the platform of Oncomine. Then, the subgroups of HCC were performed by the UALCAN website and the prognosis of HCC was analyzed by Kaplan-Meier Plotter database. Subsequently, immunohistochemistry (IHC) method was used to detect SLC41A3 expression in 323 clinically confirmed HCC samples and 184 non-cancerous liver tissues. Finally, function enrichment analysis was done using the LinkInterpreter module in LinkedOmics, and gene set enrichment analysis (GSEA) was performed using TCGA data set. The Oncomine database and immunohistochemical (IHC) showed higher SLC41A3 expression in HCC tissue compared to normal tissue. The expression of SLC41A3 was significantly correlated with tumor metastasis, Edmondson grade, microvascular invasion, and AFP level. Kaplan-Meier and Cox regression analyses verified that high SLC41A3 expression is a significant prognostic factor for reduced overall survival in HCC patients. Our results demonstrated that high expression of SLC41A3 was the predictor of poor prognosis in HCC patients, suggesting that this protein may be a potential target for HCC therapy.