Proteomic Modulation in TGF-β-Treated Cholangiocytes Induced by Curcumin Nanoparticles

• Published in: International journal of molecular sciences Vol. 24; no. 13; p. 10481
• Main Authors: Ceccherini, Elisa, Signore, Giovanni, Tedeschi, Lorena, Vozzi, Federico, Di Giorgi, Nicoletta, Michelucci, Elena, Cecchettini, Antonella, Rocchiccioli, Silvia
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 22.06.2023; MDPI
• Subjects: Analysis; Anti-Inflammatory Agents; Apoptosis; Bioavailability; Carcinogens; Cell cycle; Cell death; cholangiocytes; Comparative analysis; Curcumin; Curcumin - pharmacology; Cytokines; Data analysis; Deregulation; Fibrosis; Growth factors; Health aspects; Humans; Inflammation; Investigations; LC-MS/MS; Liver; Liver - metabolism; Mass spectra; Mediation; Membrane permeability; Metabolism; Nanoparticles; Permeability; Pharmaceutical industry; Phytochemicals; Proteins; Proteomes; Proteomics; TGF-β; Transforming Growth Factor beta - metabolism; Transforming growth factor-b; Transforming growth factors; United States; Massachusetts; Germany; LC-MS/MS; TGF-β; nanoparticles; cholangiocytes; proteomics; curcumin
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms241310481

Curcumin is a natural polyphenol that exhibits a variety of beneficial effects on health, including anti-inflammatory, antioxidant, and hepato-protective properties. Due to its poor water solubility and membrane permeability, in the present study, we prepared and characterized a water-stable, freely dispersible nanoformulation of curcumin. Although the potential of curcumin nanoformulations in the hepatic field has been studied, there are no investigations on their effect in fibrotic pathological conditions involving cholangiocytes. Exploiting an in vitro model of transforming growth factor-β (TGF-β)-stimulated cholangiocytes, we applied the Sequential Window Acquisition of All Theoretical Mass Spectra (SWATH-MS)-based quantitative proteomic approaches to study the proteome modulation induced by curcumin nanoformulation. Our results confirmed the well-documented anti-inflammatory properties of this nutraceutic, highlighting the induction of programmed cell death as a mechanism to counteract the cellular damages induced by TGF-β. Moreover, curcumin nanoformulation positively influenced the expression of several proteins involved in TGF-β-mediated fibrosis. Given the crucial importance of deregulated cholangiocyte functions during cholangiopathies, our results provide the basis for a better understanding of the mechanisms associated with this pathology and could represent a rationale for the development of more targeted therapies.