Structural Optimization of Platinum Drugs to Improve the Drug-Loading and Antitumor Efficacy of PLGA Nanoparticles

• Published in: Pharmaceutics Vol. 14; no. 11; p. 2333
• Main Authors: Sokol, Maria B, Chirkina, Margarita V, Yabbarov, Nikita G, Mollaeva, Mariia R, Podrugina, Tatyana A, Pavlova, Anna S, Temnov, Viktor V, Hathout, Rania M, Metwally, Abdelkader A, Nikolskaya, Elena D
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 29.10.2022; MDPI
• Subjects: Acids; Biological activity; Carboplatin; Chemical properties; Dosage and administration; Drug delivery systems; Drug dosages; drug release; Drugs; FDA approval; Health aspects; Ligands; molecular docking; Nanoparticles; PASS; Pharmaceutical research; Physical properties; PLGA; Polyvinyl alcohol; Severe acute respiratory syndrome coronavirus 2; Simulation; Software; Vehicles; Russia; Canada; Montreal Quebec Canada; St Louis Missouri; United States > US; Germany
• ISSN: 1999-4923; 1999-4923
• DOI: 10.3390/pharmaceutics14112333

Currently, molecular dynamics simulation is being widely applied to predict drug–polymer interaction, and to optimize drug delivery systems. Our study describes a combination of in silico and in vitro approaches aimed at improvement in polymer-based nanoparticle design for cancer treatment. We applied the PASS service to predict the biological activity of novel carboplatin derivatives. Subsequent molecular dynamics simulations revealed the dependence between the drug–polymer binding energy along with encapsulation efficacy, drug release profile, and the derivatives’ chemical structure. We applied ICP-MS analysis, the MTT test, and hemolytic activity assay to evaluate drug loading, antitumor activity, and hemocompatibility of the formulated nanoparticles. The drug encapsulation efficacy varied from 0.2% to 1% and correlated with in silico modelling results. The PLGA nanoparticles revealed higher antitumor activity against A549 human non-small-cell lung carcinoma cells compared to non-encapsulated carboplatin derivatives with IC50 values of 1.40–23.20 µM and 7.32–79.30 µM, respectively; the similar cytotoxicity profiles were observed against H69 and MCF-7 cells. The nanoparticles efficiently induced apoptosis in A549 cells. Thus, nanoparticles loaded with novel carboplatin derivatives demonstrated high application potential for anticancer therapy due to their efficacy and high hemocompatibility. Our results demonstrated the combination of in silico and in vitro methods applicability for the optimization of encapsulation and antitumor efficacy in novel drug delivery systems design.