The Impact of Hydroxytyrosol on the Metallomic-Profile in an Animal Model of Alzheimer's Disease

• Published in: International journal of molecular sciences Vol. 24; no. 19; p. 14950
• Main Authors: Tabanez, Miguel, Santos, Ilma R, Ikebara, Juliane M, Camargo, Mariana L M, Dos Santos, Bianca A, Freire, Bruna M, Batista, Bruno L, Takada, Silvia H, Squitti, Rosanna, Kihara, Alexandre H, Cerchiaro, Giselle
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 01.10.2023; MDPI
• Subjects: Advertising executives; Alzheimer Disease - chemically induced; Alzheimer Disease - drug therapy; Alzheimer's disease; Analysis; animal studies; Animals; Antioxidants; Antioxidants - adverse effects; Cobalt; Copper; Copper - pharmacology; Dementia; Disease; Disease Models, Animal; Disease susceptibility; Health aspects; Hippocampus; Humans; hydroxytyrosol; Lipids; Mass spectrometry; Maze Learning; metallomics; Neurodegeneration; Olive oil; Physiological aspects; Plasma; Rats; Rats, Wistar; Risk factors; Streptozocin - adverse effects; Trends; zinc; copper; animal studies; hydroxytyrosol; Alzheimer’s disease; zinc; metallomics
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms241914950

It is undeniable that as people get older, they become progressively more susceptible to neurodegenerative illnesses such as Alzheimer's disease (AD). Memory loss is a prominent symptom of this condition and can be exacerbated by uneven levels of certain metals. This study used inductively coupled plasma mass spectrometry (ICP-MS) to examine the levels of metals in the blood plasma, frontal cortex, and hippocampus of Wistar rats with AD induced by streptozotocin (STZ). It also tested the effects of the antioxidant hydroxytyrosol (HT) on metal levels. The Barnes maze behavior test was used, and the STZ group showed less certainty and greater distance when exploring the Barnes maze than the control group. The results also indicated that the control group and the STZ + HT group exhibited enhanced learning curves during the Barnes maze training as compared to the STZ group. The ICP-MS analysis showed that the STZ group had lower levels of cobalt in their blood plasma than the control group, while the calcium levels in the frontal cortex of the STZ + HT group were higher than in the control group. The most important finding was that copper levels in the frontal cortex from STZ-treated animals were higher than in the control group, and that the STZ + HT group returned to equivalent levels to the control group. The antioxidant HT can restore copper levels to their basal physiological state. This finding may help explain HT's potential beneficial effect in AD-patients.