B7H4 Expression Is More Frequent in MSS Status Colorectal Cancer and Is Negatively Associated with Tumour Infiltrating Lymphocytes

• Published in: Cells (Basel, Switzerland) Vol. 12; no. 6; p. 861
• Main Authors: Dawidowicz, Miriam, Kula, Agnieszka, Mielcarska, Sylwia, Kiczmer, Paweł, Skiba, Hanna, Krygier, Małgorzata, Chrabańska, Magdalena, Piecuch, Jerzy, Szrot, Monika, Robotycka, Julia, Ochman, Błażej, Strzałkowska, Bogumiła, Czuba, Zenon, Świętochowska, Elżbieta, Waniczek, Dariusz
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 01.03.2023; MDPI
• Subjects: Antigens; B7H4; Breast cancer; CD8 antigen; CD8-Positive T-Lymphocytes - pathology; Cells; Chemotherapy; Colorectal cancer; Colorectal carcinoma; Colorectal Neoplasms - pathology; CRC; Cytokines; Development and progression; Health aspects; Humans; Immune checkpoint; Immune system; Immunohistochemistry; Immunology; Immunotherapy; iTME; Lymphocytes; Lymphocytes T; Lymphocytes, Tumor-Infiltrating; Microsatellite Repeats - genetics; microsatellite stable tumours; Monoclonal antibodies; Population studies; Proteins; Reagents; Tumors; Poland; United States > US; microsatellite stable tumours; iTME; CRC; immune checkpoint; B7H4
• ISSN: 2073-4409; 2073-4409
• DOI: 10.3390/cells12060861

The immunotherapies based on ICIs in CRC are nowadays limited to microsatellite unstable tumours which are approximately 15% of all CRC cases. There are a few new immune checkpoints belonging to the B7 family, including B7H4. B7H4 expression is associated with so-called "cold tumours", and its function is linked to the downregulation of various immune cell populations. Our study aimed to investigate whether B7H4 expression is dependent on microsatellite status in CRC and on elucidating the immunological context in which the expression of B7H4 occurs. We enrolled 167 patients in the study. We prepared the homogenates from tumour tissues and healthy adjacent tissue to assess the B7H4 levels and the Bio-Plex Pro Human 48-cytokine panel. We assessed the microsatellite status of the tumour, B7H4 expression, CD8+ T cell population, and the TILs and budding in H + E stained slides by the IHC method. We used an online available database for further exploring the biological characteristics of B7H4. The expression of B7H4 was more frequent in microsatellite stable tumours, and was negatively associated with TILs. B7H4 is positively correlated with antitumour immunosuppressive iTME, thus contributing to the immunosuppressive environment in CRC.