Effect of manganese on the secretion of manganese-peroxidase by the basidiomycete Ceriporiopsis subvermispora

The ligninolytic machinery of the widely used model fungus Ceriporiopsis subvermispora includes the enzymes manganese-peroxidase (MnP) and laccase (Lcs). In this work the effect of Mn(II) on the secretion of MnP was studied. Cultures grown in the absence of Mn(II) showed high levels of mnp transcrip...

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Published inFungal genetics and biology Vol. 47; no. 7; pp. 656 - 661
Main Authors Mancilla, Rodrigo A., Canessa, Paulo, Manubens, Augusto, Vicuña, Rafael
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 01.07.2010
Subjects
Basidiomycetes
Cell culture
Ceriporiopsis subvermispora
Coriolaceae
Coriolaceae - drug effects
Coriolaceae - enzymology
Coriolaceae - genetics
culture media
cycloheximide
drug effects
enzyme activity
enzymology
Gene Expression Regulation
genetics
laccase
Laccase - genetics
Laccase - metabolism
Ligninolysis
ligninolytic microorganisms
Manganese
Manganese - pharmacology
manganese peroxidase
messenger RNA
metabolism
Microscopy, Immunoelectron
MnP
Mycelium
Mycelium - genetics
Mycelium - metabolism
nucleic acid hybridization
Peroxidases
Peroxidases - metabolism
pharmacology
Protein secretion
Western blotting
Protein secretion
Ligninolysis
MnP
Manganese
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Summary:The ligninolytic machinery of the widely used model fungus Ceriporiopsis subvermispora includes the enzymes manganese-peroxidase (MnP) and laccase (Lcs). In this work the effect of Mn(II) on the secretion of MnP was studied. Cultures grown in the absence of Mn(II) showed high levels of mnp transcripts. However, almost no MnP enzyme was detected in the extracellular medium, either by enzymatic activity assays or Western blot hybridizations. In the corresponding mycelia, immuno-electron microscopy experiments showed high levels of MnP enzyme within intracellular compartments. These results suggest that in addition to its well-known effect on transcription regulation of mnp genes, manganese influences secretion of MnP to the extracellular medium. Experiments carried out in the presence of cycloheximide confirmed that the metal is required to secrete MnP already synthesized and retained within the cell.
Bibliography:http://dx.doi.org/10.1016/j.fgb.2010.04.003
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ISSN:1087-1845
1096-0937
1096-0937
DOI:10.1016/j.fgb.2010.04.003