Aptamer-conjugated upconversion nanoprobes assisted by magnetic separation for effective isolation and sensitive detection of circulating tumor cells

Detection of circulating tumor cells （CTCs） plays an important role in cancer diagnosis and prognosis. In this study, aptamer-conjugated upconversion nano- particles （UCNPs） are used for the first time as nanoprobes to recognize tumor cells, which are then enriched by attaching with magnetic nanopar...

Full description

Saved in:
Bibliographic Details
Published inNano research Vol. 7; no. 9; pp. 1327 - 1336
Main Authors Fang, Shuai, Wang, Chao, Xiang, Jian, Cheng, Liang, Song, Xuejiao, Xu, Ligeng, Peng, Rui, Liu, Zhuang
Format Journal Article
LanguageEnglish
Published Heidelberg Tsinghua University Press 01.09.2014
Subjects
Aqueous solutions
Atomic/Molecular Structure and Spectra
Biomedicine
Biotechnology
Cancer
Centrifuges
Chemistry and Materials Science
Circulating
Condensed Matter Physics
Enrichment
Magnetic fields
Magnetic separation
Materials Science
Medical
Medical diagnosis
Nanoparticles
Nanostructure
Nanotechnology
Polyethylene glycol
Research Article
Tumors
Upconversion
上转换
循环
检测
磁性分离
纳米探针
肿瘤细胞
适体
高灵敏度
upconversion nanoparticles
circulating tumar cell (CTC) detection
magnetic nanoparticles
aptamer
Online AccessGet full text

Cover

More Information
Summary:Detection of circulating tumor cells （CTCs） plays an important role in cancer diagnosis and prognosis. In this study, aptamer-conjugated upconversion nano- particles （UCNPs） are used for the first time as nanoprobes to recognize tumor cells, which are then enriched by attaching with magnetic nanoparticles （MNPs） and placing in the presence of a magnetic field. Owing to the autofluorescence- free nature of upconversion luminescence imaging, as well as the use of magnetic separation to further reduce background signals, our technique allows for highly sensitive detection and collection of small numbers of tumor cells spiked into healthy blood samples, and shows promise for CTC detection in medical diagnostics.
Bibliography:11-5974/O4
circulating tumar cell （CTC）detection,upconversion nanoparticles,magnetic nanoparticles,aptamer
Detection of circulating tumor cells （CTCs） plays an important role in cancer diagnosis and prognosis. In this study, aptamer-conjugated upconversion nano- particles （UCNPs） are used for the first time as nanoprobes to recognize tumor cells, which are then enriched by attaching with magnetic nanoparticles （MNPs） and placing in the presence of a magnetic field. Owing to the autofluorescence- free nature of upconversion luminescence imaging, as well as the use of magnetic separation to further reduce background signals, our technique allows for highly sensitive detection and collection of small numbers of tumor cells spiked into healthy blood samples, and shows promise for CTC detection in medical diagnostics.
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 14
ObjectType-Article-1
ObjectType-Feature-2
content type line 23
ISSN:1998-0124
1998-0000
DOI:10.1007/s12274-014-0497-9