Sequence Kernel Association Tests for the Combined Effect of Rare and Common Variants

• Published in: American journal of human genetics Vol. 92; no. 6; pp. 841 - 853
• Main Authors: Ionita-Laza, Iuliana, Lee, Seunggeun, Makarov, Vlad, Buxbaum, Joseph D., Lin, Xihong
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 06.06.2013; Cell Press; Elsevier
• Subjects: Algorithms; Autism; Biological variation; Child Development Disorders, Pervasive - genetics; Computer Simulation; Crohn Disease - genetics; Crohns disease; Data Interpretation, Statistical; Gene Frequency; Genetic Association Studies - methods; Genetic Predisposition to Disease; Genetic Testing; Genomes; Humans; Logistic Models; Low Density Lipoprotein Receptor-Related Protein-2 - genetics; Models, Genetic; Nod2 Signaling Adaptor Protein - genetics; Risk; Risk assessment; Software
• ISSN: 0002-9297; 1537-6605
• DOI: 10.1016/j.ajhg.2013.04.015

Recent developments in sequencing technologies have made it possible to uncover both rare and common genetic variants. Genome-wide association studies (GWASs) can test for the effect of common variants, whereas sequence-based association studies can evaluate the cumulative effect of both rare and common variants on disease risk. Many groupwise association tests, including burden tests and variance-component tests, have been proposed for this purpose. Although such tests do not exclude common variants from their evaluation, they focus mostly on testing the effect of rare variants by upweighting rare-variant effects and downweighting common-variant effects and can therefore lose substantial power when both rare and common genetic variants in a region influence trait susceptibility. There is increasing evidence that the allelic spectrum of risk variants at a given locus might include novel, rare, low-frequency, and common genetic variants. Here, we introduce several sequence kernel association tests to evaluate the cumulative effect of rare and common variants. The proposed tests are computationally efficient and are applicable to both binary and continuous traits. Furthermore, they can readily combine GWAS and whole-exome-sequencing data on the same individuals, when available, and are also applicable to deep-resequencing data of GWAS loci. We evaluate these tests on data simulated under comprehensive scenarios and show that compared with the most commonly used tests, including the burden and variance-component tests, they can achieve substantial increases in power. We next show applications to sequencing studies for Crohn disease and autism spectrum disorders. The proposed tests have been incorporated into the software package SKAT.