Molecular characterization of resistance, virulence and clonality in vancomycin-resistant Enterococcus faecium and Enterococcus faecalis: A hospital-based study in Beijing, China

• Published in: Infection, genetics and evolution Vol. 33; pp. 253 - 260
• Main Authors: Yang, Jing-xian, Li, Tong, Ning, Yong-zhong, Shao, Dong-hua, Liu, Jing, Wang, Shu-qin, Liang, Guo-wei
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.07.2015
• Subjects: Cross Infection; Enterococcus faecalis - classification; Enterococcus faecalis - drug effects; Enterococcus faecalis - genetics; Enterococcus faecalis - pathogenicity; Enterococcus faecium - classification; Enterococcus faecium - drug effects; Enterococcus faecium - genetics; Enterococcus faecium - pathogenicity; Genotype; Gram-Positive Bacterial Infections - microbiology; Humans; Microbial Sensitivity Tests; Multilocus Sequence Typing; Vancomycin Resistance - genetics; Vancomycin-resistant enterococcus; Virulence; Virulence - genetics; Vancomycin-resistant enterococcus; Virulence; Multilocus sequence typing
• ISSN: 1567-1348; 1567-7257
• DOI: 10.1016/j.meegid.2015.05.012

•All 76 VRE strains exhibited VanA phenotype and harbored VanA gene.•Esp was the most prevalent and only virulence gene detected both in Efm and Efs.•MLST demonstrated three STs in VREfs and twelve STs in VREfm.•This is the first report of ST6 (CC2) VREfs strains in China.•This is the first outbreak report of VREfm belonging to ST571 around the world. The incidence of vancomycin-resistant enterococcus (VRE) in China is increasing, the molecular epidemiology of VRE in China is only partly known. This study was conducted to assess the molecular characterization of resistance, virulence and clonality of 69 vancomycin-resistant Enterococcus faecium (VREfm) and seven vancomycin-resistant Enterococcus faecalis (VREfs) isolates obtained from a Chinese hospital between July 2011 and July 2013. The glycopeptide resistance genes (VanA and VanB) were screened by multiplex PCR. The presence of five putative virulence genes (esp, gelE, asa1, hyl and cylA) were evaluated by another multiplex PCR. Multilocus sequence typing (MLST) scheme was used to assess the clonality. All 76 VRE isolates exhibited VanA phenotype and harbored VanA gene. Esp was the only gene detected both in VREfm and VREfs strains, accounting for 89.9% and 42.9%, respectively. The hyl gene was merely positive in 27.5% of VREfm strains. MLST analysis demonstrated three STs (ST6, ST4 and ST470) in VREfs and twelve STs (ST78, ST571, ST17, ST564, ST389, ST18, ST547, ST341, ST414, ST343, ST262 and ST203) in VREfm, which were all designated as CC17 by eBURST algorithm. An outbreak of VREfm belonging to ST571 was found to happen within the neurology ward in this hospital. To our knowledge, this is the first report of ST6 (CC2) VREfs strains in China and the first outbreak report of VREfm strains belonging to ST571 around the world. Our data could offer important information for understanding the molecular features of VRE in China.