Taiwanese and Japanese yam (Dioscorea spp.) extracts attenuate doxorubicin-induced cardiotoxicity in mice

• Published in: Yàowu shi͡p︡in fenxi Vol. 25; no. 4; pp. 872 - 880
• Main Authors: Chen, Chih-Tai, Wang, Zhi-Hong, Hsu, Cheng-Chin, Lin, Hui-Hsuan, Chen, Jing-Hsien
• Format: Journal Article
• Language: English
• Published: China (Republic : 1949- ) Elsevier B.V 01.10.2017; Food and Drug Administration; Taiwan Food and Drug Administration
• Subjects: Animal tissues; Animals; anti-inflammation; antiapoptosis; Antineoplastic Agents - toxicity; Antioxidants; Apoptosis; Apoptosis - drug effects; Blood pressure; Body weight; Cardiomyocytes; Cardiomyopathy; cardiotoxicity; Cardiotoxicity - drug therapy; Cardiotoxicity - etiology; Cardiotoxicity - genetics; Cardiotoxicity - metabolism; Caspase; Caspase-3; Chemotherapy; Cultivars; Cytochrome; Dioscorea; Dioscorea - chemistry; Dioscorea alata; Dioscorea japonica; Doxorubicin; Doxorubicin - toxicity; Enzymes; Ethanol; Glutathione; Glutathione peroxidase; Glutathione Peroxidase - metabolism; Heart; Heart diseases; Heart rate; Humans; In vivo methods and tests; Inflammation; Japan; L-Lactate dehydrogenase; Laboratory animals; Lactate dehydrogenase; Lactic acid; Lipid peroxidation; Lipids; Male; Mice; Mice, Inbred BALB C; Mitochondria; Moisture absorption; Original; Oxidative stress; Oxidative Stress - drug effects; Peroxidase; Plant Extracts - administration & dosage; Property damage; Proteins; Reactive oxygen species; Reactive Oxygen Species - metabolism; Studies; Superoxide dismutase; Superoxide Dismutase - metabolism; Taiwan; Thiobarbituric acid; yam; Taiwan; cardiotoxicity; anti-inflammation; doxorubicin; yam; antiapoptosis
• ISSN: 1021-9498; 2224-6614
• DOI: 10.1016/j.jfda.2016.09.002

The present study was designed to explore whether yam could protect the heart from doxorubicin (DOX)-induced oxidative stress leading to cardiotoxicity in vivo. In this study, the protective effects of water and ethanol extracts of three varieties of yam, including water extracts of Dioscorea japonica Thunb., ethanol extracts of D. japonica Thunb., water extracts of Dioscorea alata, ethanol extracts of D. alata, water extracts of Dioscorea purpurea, and ethanol extracts of D. purpurea, against DOX-induced cardiotoxicity in experimental mice were evaluated. DOX treatment led to significant decreases in the ratio of heart weight to body weight and heart rate, and increases in blood pressure and the serum level of lactate dehydrogenase, a marker of cardiotoxicity, were recovered by yam extracts, especially in water extracts of D. alata. Yam extracts also decreased the cardiac levels of thiobarbituric acid relative substances, reactive oxygen species, and inflammatory factors, as well as the expression of nuclear factor kappa B, while ethanol extracts of D. japonica Thunb. and D. purpurea were shown to be more potent. Moreover, yam extracts had a role in increasing the activities of glutathione peroxidase and superoxide dismutase, thus improving the DOX-induced alterations in oxidative status in the heart tissue of DOX-treated mice. All ethanol extracts of yam exhibited their antiapoptotic abilities on caspase-3 activation and mitochondrial dysfunction, and ethanol extracts of D. alata still exerted a superior effect. Based on these findings, it can be concluded that yam has significant cardioprotective properties against DOX-induced damage via its multiple effects on antioxidant, anti-inflammatory, or antiapoptotic activities. AE=ethanol extracts of D. alata; AW=water extracts of D. alata (Ta-Shan); JE=ethanol extracts of D. japonica Thunb.; JW=water extracts of D. japonica Thunb.; LDH=lactate dehydrogenase; PE=ethanol extracts of D. purpurea; PW=water extracts of D. purpurea. [Display omitted]