Genome Editing and Cardiac Arrhythmias

• Published in: Cells (Basel, Switzerland) Vol. 12; no. 10; p. 1363
• Main Authors: Moore, Oliver M, Ho, Kevin S, Copeland, Juwan S, Parthasarathy, Vaidya, Wehrens, Xander H T
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 11.05.2023; MDPI
• Subjects: Arrhythmia; arrhythmias; Arrhythmias, Cardiac - genetics; Arrhythmias, Cardiac - metabolism; Arrhythmias, Cardiac - therapy; Cardiac arrhythmia; Cardiomyocytes; Cardiomyopathy; Cardiovascular disease; Care and treatment; catecholaminergic polymorphic ventricular tachycardia; Coronary vessels; CRISPR; CRISPR/Cas9; Efficiency; Gene Editing; Gene therapy; Gene transfer; Genetic aspects; Genome editing; Genomes; Heart; Heart cells; Humans; long QT syndrome; Muscular dystrophy; Mutation; Myocytes, Cardiac - metabolism; Physiological aspects; Review; Reviews; ryanodine receptor; Tachycardia, Ventricular - genetics; Tachycardia, Ventricular - metabolism; Tachycardia, Ventricular - therapy; Vein & artery diseases; United States; RyR2; genome editing; catecholaminergic polymorphic ventricular tachycardia; CRISPR/Cas9; arrhythmias; long QT syndrome; ryanodine receptor
• ISSN: 2073-4409; 2073-4409
• DOI: 10.3390/cells12101363

This article reviews progress in the field of cardiac genome editing, in particular, its potential utility in treating cardiac arrhythmias. First, we discuss genome editing methods by which DNA can be disrupted, inserted, deleted, or corrected in cardiomyocytes. Second, we provide an overview of in vivo genome editing in preclinical models of heritable and acquired arrhythmias. Third, we discuss recent advancements in cardiac gene transfer, including delivery methods, gene expression optimization, and potential adverse effects associated with therapeutic somatic genome editing. While genome editing for cardiac arrhythmias is still in its infancy, this approach holds great promise, especially for inherited arrhythmia syndromes with a defined genetic defect.