Maturation of dendritic cells by bacterial immunomodulators

• Published in: Vaccine Vol. 22; no. 21; pp. 2761 - 2768
• Main Authors: Spisek, Radek, Brazova, Jitka, Rozkova, Daniela, Zapletalova, Katerina, Sediva, Anna, Bartunkova, Jirina
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 29.07.2004; Elsevier; Elsevier Limited
• Subjects: Adjuvants, Immunologic - chemistry; Adjuvants, Immunologic - pharmacology; Antibody Formation - drug effects; Antigens, Surface - analysis; Applied microbiology; Bacteria; Bacteria - chemistry; Bacterial immunomodulators; Bacteriology; Biological and medical sciences; Cells, Cultured; Clinical trials; Culture Media; Cytokines - analysis; Cytokines - biosynthesis; Dendritic cell; Dendritic Cells - drug effects; Dextrans; Endocytosis; Flow Cytometry; Fluorescein-5-isothiocyanate; Fundamental and applied biological sciences. Psychology; Humans; Immune system; Immunotherapy; Interleukin-10 - biosynthesis; Interleukin-12 - biosynthesis; Lymphocyte Culture Test, Mixed; Lymphocytes; Maturation; Microbiology; Miscellaneous; Phagocytosis - drug effects; Phenotype; Reproducibility of Results; Stimulation, Chemical; Streptococcus infections; T-Lymphocytes - drug effects; T-Lymphocytes - immunology; Vaccines; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects); Dendritic cell; Maturation; Bacterial immunomodulators; Immunotherapy; Immunomodulator; Bacteria
• ISSN: 0264-410X; 1873-2518
• DOI: 10.1016/j.vaccine.2004.01.006

Dendritic cells (DC) become fully functional upon maturation by various stimuli. We tested whether an immunostimulatory effect of clinically used immunomodulators (Luivac ®, Biostim ®, Ribomunyl ®, Imudon ®, Bronchovaxom ®) is caused by direct DC activation. We found that Luivac ®, Biostim ® and Ribomunyl ® have a very high DC stimulatory potential in vitro. The level of DC activation was comparable or higher than DC maturation induced by standard maturation stimuli, Poly (I:C) or lipopolysaccharide. Treated DC had activated phenotype, reduced phagocytic activity and they induced the proliferation of allogeneic T lymphocytes. These results are important for understanding the physiology of action of these widely prescribed agents. Administration of bacterial immunomodulators should be considered with care to avoid the potential risk of inducing an autoimmune disease. They could also be used as well-defined maturating agents in the protocols used for the ex vivo production of DC-based vaccines for clinical trials.