Seminal immunoreactive relaxin in domestic animals and its relationship to sperm motility as a possible index for predicting the fertilizing ability of sires

• Published in: International journal of andrology Vol. 26; no. 2; pp. 115 - 120
• Main Authors: Kohsaka, T., Hamano, K., Sasada, H., Watanabe, S., Ogine, T., Suzuki, E., Nishida, S., Takahara, H., Sato, E.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Science Ltd 01.04.2003; Blackwell
• Subjects: Animals; Biological and medical sciences; Cattle; domestic animals; Fertility; Fluoroimmunoassay; Fundamental and applied biological sciences. Psychology; Goats; Humans; Male; motility; relaxin; Relaxin - analysis; Relaxin - physiology; Semen - chemistry; seminal plasma; Sheep; sperm; Sperm Motility - physiology; domestic animals; motility; seminal plasma; relaxin; sperm
• ISSN: 0105-6263; 1365-2605
• DOI: 10.1046/j.1365-2605.2003.00409.x

Although immunoassayable relaxin has been detected in human and boar seminal plasma, there is no evidence suggesting the existence of immunoreactive relaxin in the seminal plasma of other domestic animals. The first objective of this study was to determine whether immunoreactive relaxin was present in the seminal plasma of bulls, rams and he‐goats. In addition, the correlation of immunoreactive relaxin with sperm motility as an index for predicting the fertilizing ability of bull sires was investigated. Semen with normal sperm motility was collected from bulls, rams and he‐goats, and the relaxin immunoreactivity of the semen samples was measured using a time‐resolved fluoroimmunoassay (TR‐FIA) for porcine relaxin that we developed. The presence of relaxin immunoreactivity was demonstrated in seminal plasma from bulls, rams and he‐goats. The level of immunoreactive relaxin in seminal plasma was the highest in bulls followed by humans, rams, boars and he‐goats in that order, when relaxin levels in boar and human semen having normal sperm motility were also assayed under the same conditions. When the correlation between the seminal plasma level of immunoreactive relaxin and sperm motility was examined in bull semen samples as an index for predicting fertilizing ability, it was found that the relaxin level was significantly correlated with the percentage of spermatozoa showing the most intensive motility (r = 0.64, p < 0.05). These results indicate that immunoreactive relaxin is widely found in the seminal plasma of domestic animals and that measuring the relaxin concentration of seminal plasma may be useful to identify subfertile sires or predict the fertility potential of individual sires.