Cowpea-Meloidogyne incognita interaction: Root proteomic analysis during early stages of nematode infection

• Published in: Proteomics (Weinheim) Vol. 15; no. 10; pp. 1746 - 1759
• Main Authors: Villeth, Gabriela R. C., Carmo, Lilian S. T., Silva, Luciano P., Fontes, Wagner, Grynberg, Priscila, Saraiva, Mario, Brasileiro, Ana C. M., Carneiro, Regina M. D., Oliveira, José T. A., Grossi-de-Sá, Maria F., Mehta, Angela
• Format: Journal Article
• Language: English
• Published: Germany Blackwell Publishing Ltd 01.05.2015; Wiley Subscription Services, Inc
• Subjects: 2-DE; Animals; Biosynthesis; Cultivars; Differential expression; Drought; Electrophoresis, Gel, Two-Dimensional; Endopeptidase; Fabaceae - genetics; Fabaceae - parasitology; Fertility; Gene expression; Gene Expression Profiling; Gene Expression Regulation, Plant; Genetic Association Studies; Glyceraldehyde-3-phosphate dehydrogenase; Host-Parasite Interactions - genetics; Infections; Inoculation; Mass Spectrometry; Meloidogyne incognita; Nematoda; Nematode-plant interaction; Oxidative phosphorylation; Oxidative stress; Phosphorylation; Plant Diseases - genetics; Plant Diseases - parasitology; Plant Proteins - genetics; Plant Proteins - metabolism; Plant proteomics; Plant Roots - metabolism; Plant Roots - parasitology; Proteasomes; Proteins; Proteomics - methods; RNA, Messenger - genetics; RNA, Messenger - metabolism; Soil fertility; Spermidine; Spermidine synthase; Tylenchoidea - physiology; Ubiquitination; Vigna unguiculata; Plant proteomics; 2-DE; Nematode-plant interaction; Differential expression; MS
• ISSN: 1615-9853; 1615-9861
• DOI: 10.1002/pmic.201400561

Cowpea (Vigna unguiculata L. Walp) is an important legume species well adapted to low fertility soils and prolonged drought periods. One of the main problems that cause severe yield losses in cowpea is the root‐knot nematode Meloidogyne incognita. The aim of this work was to analyze the differential expression of proteins in the contrasting cultivars of cowpea CE 31 (highly resistant) and CE 109 (slightly resistant) during early stages of M. incognita infection. Cowpea roots were collected at 3, 6, and 9 days after inoculation and used for protein extraction and 2‐DE analysis. From a total of 59 differential spots, 37 proteins were identified, mostly involved in plant defense, such as spermidine synthase, patatin, proteasome component, and nitrile‐specifier protein. A follow‐up study was performed by quantitative RT‐PCR analysis of nine selected proteins and the results revealed a very similar upregulation trend between the protein expression profiles and the corresponding transcripts. This study also identified ACT and GAPDH as a good combination of reference genes for quantitative RT‐PCR analysis of the pathosystem cowpea/nematode. Additionally, an interactome analysis showed three major pathways affected by nematode infection: proteasome endopeptidase complex, oxidative phosphorylation, and flavonoid biosynthesis. Taken together, the results obtained by proteome, transcriptome, and interactome approaches suggest that oxidative stress, ubiquitination, and glucosinolate degradation may be part of cowpea CE 31 resistance mechanisms in response to nematode infection.