Overexpression of a FAD3 Desaturase Increases Synthesis of a Polymethylene-Interrupted Dienoic Fatty Acid in Seeds of Arabidopsis thaliana L

• Published in: Lipids Vol. 44; no. 8; pp. 753 - 757
• Main Authors: Puttick, Debbie, Dauk, Melanie, Lozinsky, Sharla, Smith, Mark A
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Berlin/Heidelberg : Springer-Verlag 01.08.2009; Springer-Verlag; Springer‐Verlag; Springer Nature B.V
• Subjects: Arabidopsis; Arabidopsis - genetics; Arabidopsis - metabolism; Arabidopsis Proteins - genetics; Arabidopsis Proteins - physiology; Arabidopsis thaliana; Biomedical and Life Sciences; Communication; dienoic fatty acids; Fatty Acid Desaturases - genetics; Fatty Acid Desaturases - physiology; Fatty acids; Fatty Acids, Unsaturated - biosynthesis; Fatty Acids, Unsaturated - chemistry; Gas chromatography; Gene Expression Regulation, Enzymologic; Gene Expression Regulation, Plant; gene overexpression; genetic transformation; Life Sciences; Linoleate desaturase; Linoleic Acids - biosynthesis; linolenic acid; Lipid Metabolism - genetics; Lipidology; Mangiferic acid; Medical Biochemistry; Medicinal Chemistry; Methane - analogs & derivatives; Methane - chemistry; Microbial Genetics and Genomics; Neurochemistry; Nutrition; Oils & fats; oleic acid; Plants, Genetically Modified; Polymethylene‐interrupted dienoic fatty acid; polyunsaturated fatty acids; Seed oil; seed oils; Seeds; Seeds - genetics; Seeds - metabolism; Spectrometry; stearoyl-CoA desaturase; Transfection; transgenes; transgenic plants; Up-Regulation - physiology; Linoleate desaturase; Seed oil; Polymethylene-interrupted dienoic fatty acid; Mangiferic acid
• ISSN: 0024-4201; 1558-9307
• DOI: 10.1007/s11745-009-3315-5

A cDNA encoding the Arabidopsis extraplastidic linoleate desaturase (FAD3) was overexpressed in the seeds of wild-type Arabidopsis and in a mutant line that accumulates high levels of oleic acid. In the transformed wild-type plants, linolenic acid (18:3Δ9,12,15) increased from 19% to nearly 40% of total seed fatty acids, with a corresponding decrease in linoleate content (18:2Δ9,12). In the high oleate mutant, a large increase in the level of a fatty acid identified by gas-chromatography/mass-spectrometry as mangiferic acid (18:2Δ9,15) was observed. The results demonstrate that the polymethylene-interrupted dienoic fatty acid, mangiferic acid, can be produced in seed oil through the overexpression of a fatty acid n-3 desaturase.