Climbing-fibre activation of NMDA receptors in Purkinje cells of adult mice
Among principal neurons, adult Purkinje cells have long been considered unusual in lacking functional NMDA receptors. This view has emerged largely from studies on rats, where NMDA receptors are expressed in Purkinje cells of newborn animals, but are lost after 2 weeks. By contrast, immunolabelling...
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Published in | The Journal of physiology Vol. 585; no. 1; pp. 91 - 101 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
Oxford, UK
The Physiological Society
15.11.2007
Blackwell Publishing Ltd |
Subjects | |
Online Access | Get full text |
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Summary: | Among principal neurons, adult Purkinje cells have long been considered unusual in lacking functional NMDA receptors. This
view has emerged largely from studies on rats, where NMDA receptors are expressed in Purkinje cells of newborn animals, but
are lost after 2 weeks. By contrast, immunolabelling data have shown that Purkinje cells from adult mice express multiple
NMDA receptor subunits, suggesting a possible species difference. To investigate the presence of functional NMDA receptors
in Purkinje cells of mice, and to explore the contribution of different receptor subunits, we made whole-cell and single-channel
patch-clamp recordings from Purkinje cells of wild-type and NR2Dâ/â mice of different ages. Here we report that multiple NMDA
receptor subtypes are indeed expressed in Purkinje cells of young and adult mice; in the adult, both NR2A- and NR2B-containing
subtypes are present. Furthermore, we show that NMDA receptor-mediated EPSCs can be evoked by climbing fibre stimulation,
and appear to be mediated mainly by NR2A-containing receptors. |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 |
ISSN: | 0022-3751 1469-7793 |
DOI: | 10.1113/jphysiol.2007.141531 |