A Comparative Study of Fibroblast Behaviors under Cyclic Stress Stimulus and Static Culture on 3D Patterned Matrix

• Published in: Journal of bionics engineering Vol. 10; no. 2; pp. 148 - 155
• Main Authors: Zhao, Mingyan, Li, Lihua, Li, Bo, Wu, Di, Zhou, Changren, Tian, Ye
• Format: Journal Article
• Language: English
• Published: Singapore Elsevier Ltd 01.04.2013; Springer Singapore
• Subjects: Artificial Intelligence; bFGF; Biochemical Engineering; Bioinformatics; Biomaterials; Biomedical Engineering and Bioengineering; Biomedical Engineering/Biotechnology; Chitosan; Engineering; fibroblast; Fibroblasts; Honeycomb; Honeycomb construction; mechanical stimulus; polymer composites; Scaffolds; Secretions; Stresses; Three dimensional; 图案化; 应力刺激; 循环; 矩阵; 碱性成纤维细胞生长因子; 细胞行为; 静态培养; fibroblast; polymer composites; bFGF; mechanical stimulus
• ISSN: 1672-6529; 2543-2141
• DOI: 10.1016/S1672-6529(13)60209-7

Mechanical stress and patterned surface of the scaffolds has been recognized as a crucial factor in determining cell func- tionality and tissue development, which in turn can direct the cell responses. In this study, fibroblasts M-3T3 in three-dimensional （3D） honeycomb patterning Chitosan/Poly（L-Lactic Acid） （CS/PLLA） composites was stimulated by a 15% sinusoidal （1 Hz） strain applied by a biodynamic test instrument. The effects of mechanical stimulus on the cell proliferation and basic Fibroblast Growth Factor （bFGF） secretion were studied in comparison to the non-strain groups and blank control. Results show that fibroblasts are able to sense the mechanical stimulation and respond, resulting in a time dependent increase of bFGF secretion and promoting cell proliferation. Moreover, the cells seeded in the scaffolds showed a higher cell proliferation and bFGF secretion. These findings support the hypothesis that suitable mechanical stimulus has positive effect on fibroblasts, and such a 3D honeycomb patterned scaffold may play a positive role in regulating cell behaviors in vitro.