The effects of pH on carbon material and energy balances in hydrogen-producing Clostridium tyrobutyricum JM1

• Published in: Bioresource technology Vol. 99; no. 17; pp. 8485 - 8491
• Main Authors: Jo, Ji Hye, Lee, Dae Sung, Park, Jong Moon
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 01.11.2008; [New York, NY]: Elsevier Ltd; Elsevier
• Subjects: Anaerobiosis; Biological and medical sciences; Carbon - metabolism; Carbon material balance; Clostridium tyrobutyricum; Clostridium tyrobutyricum - metabolism; Energy balance; Energy Metabolism; Fermentation; Fundamental and applied biological sciences. Psychology; Glucose - metabolism; Hydrogen - metabolism; Hydrogen production; Hydrogen-Ion Concentration; pH effect; Energy balance; Carbon material balance; pH effect; Hydrogen production; Clostridium tyrobutyricum; Hydrogen; Clostridiales; Carbon; Carbon balance; Clostridiaceae; Production; pH; Bacteria; Material balance
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2008.03.060

The effects of pH on hydrogen fermentation of glucose by newly isolated H 2-producing bacterium Clostridium tyrobutyricum JM1 were investigated in batch cultivations. The changes of carbon material and energy balances by pH conditions provided useful information for understanding and interpreting the regulatory system of the microorganism, and for optimization of a desired product, in this case, molecular hydrogen. The most probable metabolic pathways of C. tyrobutyricum JM1 were determined through an accurate analysis of stoichiometry and the consistency of the experimental data, checked by high carbon recovery. The carbon material and energy balances were adequately applied to estimate the carbon-flow distribution. They suggested that pH 6.3 was appropriate to maximize hydrogen production with a high concentration of butyrate and balanced activities of NADH.