Synthesis of Magneto-Controllable Polymer Nanocarrier Based on Poly(N-isopropylacrylamide-co-acrylic Acid) for Doxorubicin Immobilization

• Published in: Polymers Vol. 14; no. 24; p. 5440
• Main Authors: Kusaia, Viktoria S, Kozhunova, Elena Yu, Stepanova, Darya A, Pigareva, Vladislava A, Sybachin, Andrey V, Zezin, Sergey B, Bolshakova, Anastasiya V, Shchelkunov, Nikita M, Vavaev, Evgeny S, Lyubin, Evgeny V, Fedyanin, Andrey A, Spiridonov, Vasiliy V
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 01.12.2022; MDPI
• Subjects: Acrylic acid; Active control; Antimitotic agents; Antineoplastic agents; Aqueous solutions; Atomic force microscopy; Biocompatibility; Cancer therapies; Cell membranes; Chemical synthesis; Controllability; controlled delivery; Diffraction; Doxorubicin; Fluorescence microscopy; Hemodialysis; Infrared spectroscopy; Iron; Isopropylacrylamide; Magnetic fields; Magnetic properties; magnetosensitivity; microgel; Microgels; Microscopy; model cell membranes; Molecular weight; Mossbauer spectroscopy; Nanoparticles; optical tweezers; Pharmaceuticals; Photon correlation spectroscopy; Physiological aspects; Polymer industry; Polymerization; Polymers; Spectrum analysis; Stability; static light scattering; Surface active agents; Surfactants; X-rays; Russia; St Louis Missouri; United States > US; microgel; controlled delivery; magnetosensitivity; static light scattering; optical tweezers; doxorubicin; model cell membranes
• ISSN: 2073-4360; 2073-4360
• DOI: 10.3390/polym14245440

In this work, the preparation procedure and properties of anionic magnetic microgels loaded with antitumor drug doxorubicin are described. The functional microgels were produced via the in situ formation of iron nanoparticles in an aqueous dispersion of polymer microgels based on poly(N-isopropylacrylamide-co-acrylic acid) (PNIPAM-PAA). The composition and morphology of the resulting composite microgels were studied by means of X-ray diffraction, Mössbauer spectroscopy, IR spectroscopy, scanning electron microscopy, atomic-force microscopy, laser microelectrophoresis, and static and dynamic light scattering. The forming nanoparticles were found to be β-FeO(OH). In physiological pH and ionic strength, the obtained composite microgels were shown to possess high colloid stability. The average size of the composites was 200 nm, while the zeta-potential was -27.5 mV. An optical tweezers study has demonstrated the possibility of manipulation with microgel using external magnetic fields. Loading of the composite microgel with doxorubicin did not lead to any change in particle size and colloidal stability. Magnetic-driven interaction of the drug-loaded microgel with model cell membranes was demonstrated by fluorescence microscopy. The described magnetic microgels demonstrate the potential for the controlled delivery of biologically active substances.