Synthetic promoters consisting of defined cis-acting elements link multiple signaling pathways to probenazole-inducible system

• Published in: Journal of Zhejiang University. B. Science Vol. 16; no. 4; pp. 253 - 263
• Main Authors: Zhu, Zheng, Gao, Jiong, Yang, Jin-xiao, Wang, Xiao-yan, Ren, Guo-dong, Ding, Yu-long, Kuai, Ben-ke
• Format: Journal Article
• Language: English
• Published: Hangzhou Zhejiang University Press 01.04.2015; Springer Nature B.V
• Subjects: Arabidopsis - drug effects; Arabidopsis - genetics; Biomedical and Life Sciences; Biomedicine; Biosynthesis; Calcium influx; Calcium ions; Calcium signalling; Ethylene; Genes, Synthetic; Innate immunity; Jasmonic acid; Kinases; MAP kinase; Plant Diseases - genetics; Plant Diseases - prevention & control; Plant Proteins - genetics; Plants, Genetically Modified - drug effects; Plants, Genetically Modified - genetics; Probenazole; Promoter Regions, Genetic - genetics; Promoters; Protein kinase; Regulatory Elements, Transcriptional - genetics; Reporter gene; Salicylic acid; Signal transduction; Signal Transduction - drug effects; Signal Transduction - genetics; Signaling; Thiazoles - pharmacology; Q789; Chemical-inducible system; Probenazole; Systemic acquired resistance (SAR); Synthetic promoter; 人工合成启动子; 顺式作用元件; 化学诱导系统; acting element; 烯丙异噻唑; Cis-acting element
• ISSN: 1673-1581; 1862-1783
• DOI: 10.1631/jzus.B1400203

Probenazole (3-allyloxy-1,2-benzisothiazole-1,1-dioxide, PBZ), the active component of Oryzemate, could induce systemic acquired resistance (SAR) in plants through the induction of salicylic acid (SA) biosynthesis. As a widely used chemical inducer, PBZ is a good prospect for establishing a new chemical-inducible system. We first designed artificially synthetic promoters with tandem copies of a single type of cis -element (SARE, JERE, GCC, GST1, HSRE, and W-box) that could mediate the expression of the β-glucuronidase ( GUS ) reporter gene in plants upon PBZ treatment. Then we combined different types of elements in order to improve inducibility in the PBZ-inducible system. On the other hand, we were surprised to find that the cis -elements, which are responsive to jasmonic acid (JA) and ethylene, also responded to PBZ, implying that SA, JA, and ethylene pathways also would play important roles in PBZ’s action. Further analysis demonstrated that PBZ also induced early events of innate immunity via a signaling pathway in which Ca 2+ influx and mitogen-activated protein kinase (MAPK) activity were involved. We constructed synthesized artificial promoters to establish a PBZ chemical-inducible system, and preliminarily explored SA, JA, ethylene, calcium, and MAPK signaling pathways via PBZ-inducible system, which could provide an insight for in-depth study.