miR-143-3p regulates cell proliferation and apoptosis by targeting IGF1R and IGFBP5 and regulating the Ras/p38 MAPK signaling pathway in rheumatoid arthritis

• Published in: Experimental and therapeutic medicine Vol. 15; no. 4; pp. 3781 - 3790
• Main Authors: Yang, Zhenguo, Wang, Jifu, Pan, Zhuangzhuang, Zhang, Yihang
• Format: Journal Article
• Language: English
• Published: Greece Spandidos Publications 01.04.2018; Spandidos Publications UK Ltd; D.A. Spandidos
• Subjects: Age; Apoptosis; Care and treatment; Cell growth; Cellular signal transduction; Cytokines; Development and progression; Fibroblasts; Gene expression; Genetic aspects; Health aspects; Inflammation; Inflammatory diseases; Insulin-like growth factors; Joint surgery; Kinases; Ligands; MicroRNA; Pathogenesis; Patients; Rheumatoid arthritis; Studies; Tumor necrosis factor-TNF; United States > US; insulin-like growth factor binding protein 5; insulin-like growth factor 1 receptor; synovial fibroblasts; microRNA-143-3p; rheumatoid arthritis
• ISSN: 1792-0981; 1792-1015
• DOI: 10.3892/etm.2018.5907

It has been demonstrated that the deregulation of microRNAs (miRNAs) affects the development of rheumatoid arthritis (RA). The primary objective of the current study was to determine the role of miR-143-3p in the progression of RA. The expression of miR-143-3p in synovium taken from patients with RA was assessed by reverse transcription-quantitative polymerase chain reaction. The expression of miR-143-3p was higher in synovium tissues of RA than that of osteoarthritis (OA). The decreased expression of miR-143-3p suppressed cell proliferation and promoted apoptosis . In addition, inhibition of miR-143-3p decreased levels of inflammatory cytokines, as determined by an enzyme-linked immunosorbent assay. IGF1R and IGFBP5 were found to be the target genes of miR-143-3p, and it was demonstrated that miR-143-3p regulated the proliferation and apoptosis of MH7A cells by targeting IGF1R and IGFBP5. Furthermore, TNF-α treatment stimulated the Ras/p38 mitogen activated protein kinase (MAPK) signaling pathway, whereas miR-143-3p inhibition suppressed it. The results of the current study indicate that miR-143-3p may regulate cell proliferation and apoptosis by targeting IGF1R and IGFBP5 expression and regulating the Ras/p38 MAPK signaling pathways. Therefore, miR-143-3p may be a novel therapeutic target in RA.