LncRNA 148400 Promotes the Apoptosis of Renal Tubular Epithelial Cells in Ischemic AKI by Targeting the miR-10b-3p/GRK4 Axis

• Published in: Cells (Basel, Switzerland) Vol. 11; no. 24; p. 3986
• Main Authors: Li, Xingjin, Wu, Zhifen, Yang, Jurong, Zhang, Dongshan
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 01.12.2022; MDPI
• Subjects: Acute Kidney Injury - genetics; Acute Kidney Injury - metabolism; Acute Kidney Injury - pathology; Acute renal failure; AKI; Analysis; Animals; Apoptosis; Apoptosis - genetics; Apoptosis - physiology; Caspase; Creatinine; Epithelial cells; Epithelial Cells - metabolism; Epithelial Cells - pathology; G proteins; Gene expression; GRK4; Health aspects; Ischemia; Ischemia - genetics; Ischemia - metabolism; Kidney Tubules, Proximal - blood supply; Kidney Tubules, Proximal - metabolism; Kidney Tubules, Proximal - pathology; Kidneys; lncRNA148400; Mice; Mice, Inbred C57BL; MicroRNA; MicroRNAs - genetics; MicroRNAs - metabolism; miR−10b−3p; Morphology; Nitrogen; Non-coding RNA; Prevention; Renal function; Risk factors; RNA, Long Noncoding - genetics; China; United States > US; apoptosis; miR−10b−3p; GRK4; lncRNA148400; AKI
• ISSN: 2073-4409; 2073-4409
• DOI: 10.3390/cells11243986

Although recent studies have reported that long non-coding RNA (lncRNA) is involved in the development of ischemic acute kidney injury (AKI), the exact function and regulatory mechanism of lncRNAs in ischemic AKI remain largely unknown. Herein, we found that ischemic injury promoted the expression of lncRNA 148400 in mouse proximal tubule-derived cell line (BUMPT) and C57BL/6J mice. Furthermore, the lncRNA148400 mediates ischemic injury-induced apoptosis of BUMPT cells. Mechanistically, lncRNA 148400 sponged miR-10b-3p to promote apoptosis via GRK4 upregulation. Finally, knockdown of lncRNA 148400 alleviated the I/R-induced deterioration of renal function, renal tubular injury, and cell apoptosis. In addition, cleaved caspase-3 is increased via targeting the miR-10b-3p/GRK4 axis. Collectively, these results showed that lncRNA 148400/miR-10b-3p/GRK4 axis mediated the development of ischemic AKI.