Poly(ethylene oxide)-bonded stationary phase for separation of inorganic anions in capillary ion chromatography

• Published in: Journal of Chromatography A Vol. 1294; pp. 117 - 121
• Main Authors: Linda, Roza, Lim, Lee Wah, Takeuchi, Toyohide
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 14.06.2013; Elsevier
• Subjects: acetonitrile; Analytical chemistry; anion exchange; Anions; Anions - chemistry; Applied sciences; Biological and medical sciences; Cations; Chemistry; Chromatographic methods and physical methods associated with chromatography; Chromatography; Chromatography, Ion Exchange - instrumentation; Chromatography, Ion Exchange - methods; Drinking water and swimming-pool water. Desalination; Eluents; Elution; ethylene oxide; Exact sciences and technology; Inorganic anions; Investigative techniques, diagnostic techniques (general aspects); Iodates; iodides; Ion chromatography; Ion Exchange; ion exchange chromatography; Medical sciences; Miscellaneous. Technology; nitrates; Other chromatographic methods; packaging materials; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques; Pollution; Polyethylene Glycols - chemistry; Retention behavior; Separation; sodium chloride; Standard deviation; Tosylated-poly(ethylene oxide); Water treatment and pollution; Retention behavior; Ion chromatography; Tosylated-poly(ethylene oxide); Inorganic anions; Capillary column; Biological fluid; Chemical analysis; Tap water; Ethylene oxide polymer; Chemical modification; Inorganic anion; Ultraviolet detector; Clinical biology; Water quality; Water pollution; Saliva; Tosylation; Bonded stationary phase
• ISSN: 0021-9673; 1873-3778
• DOI: 10.1016/j.chroma.2013.04.052

•PEO-bonded stationary phase was formed by a single and simple step reaction.•Anions were successfully separated by ion exchange interaction.•Eluent cation was coordinated among the PEO chains and worked as the ion-exchange site.•Elution order was similar as those observed in common ion exchange chromatography.•Cations and anions of the eluent affected the retention of sample anions. A tosylated-poly(ethylene oxide) (PEO) reagent was reacted with primary amino groups of an aminopropylsilica packing material (TSKgel NH2-60) in acetonitrile to form PEO-bonded stationary phase. The reaction was a single and simple step reaction. The prepared stationary phase was able to separate inorganic anions. The retention behavior of six common inorganic anions on the prepared stationary phase was examined under various eluent conditions in order to clarify its separation/retention mechanism. The elution order of the tested anions was iodate, bromate, bromide, nitrate, iodide, and thiocyanate, which was similar as observed in common ion chromatography. The retention of inorganic anions could be manipulated by ion exchange interaction which is expected that the eluent cation is coordinated among the PEO chains and it works as the anion-exchange site. Cations and anions of the eluent therefore affected the retention of sample anions. We demonstrated that the retention of the analyte anions decreased with increasing eluent concentration. The repeatability of retention time for the six anions was satisfactory on this column with relative standard deviation values from 1.1 to 4.3% when 10mM sodium chloride was used as the eluent. Compared with the unmodified TSKgel NH2-60, the prepared stationary phase retained inorganic anions more strongly and the selectivity was also improved. The present stationary phase was applied for the determination of inorganic anions contained in various water samples.