Bioinformatics Analysis of the Inflammation-Associated lncRNA-mRNA Coexpression Network in Type 2 Diabetes

Introduction. Diabetes is a chronic inflammatory state, and a key role of lncRNAs in diabetes complications is a new area of research. Methods. In this study, key lncRNAs related to diabetes inflammation were identified by RNA-chip mining and lncRNA-mRNA coexpression network construction and finally...

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Published inJournal of the renin-angiotensin-aldosterone system Vol. 2023; p. 6072438
Main Authors Huang, Linjuan, Xiong, Shengxi, Liu, Hanshuang, Li, Min, Zhang, Ranran, Liu, Yan, Hu, Xiaolei
Format Journal Article
LanguageEnglish
Published England Hindawi 01.01.2023
Hindawi Limited
Subjects
Bioinformatics
Biomarkers
Computational Biology
Diabetes
Diabetes Mellitus, Type 2
Endocrine system
Genes
Humans
Inflammation
Lipopolysaccharides
RNA, Long Noncoding
RNA, Messenger
Sodium-Potassium-Exchanging ATPase
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Summary:Introduction. Diabetes is a chronic inflammatory state, and a key role of lncRNAs in diabetes complications is a new area of research. Methods. In this study, key lncRNAs related to diabetes inflammation were identified by RNA-chip mining and lncRNA-mRNA coexpression network construction and finally verified by RT-qPCR. Results. We ultimately obtained 12 genes, including A1BG-AS1, AC084125.4, RAMP2-AS1, FTX, DBH-AS1, LOXL1-AS1, LINC00893, LINC00894, PVT1, RUSC1-AS1, HCG25, and ATP1B3-AS1. RT-qPCR assays verified that LOXL1-AS1, A1BG-AS1, FTX, PVT1, and HCG25 were upregulated in the HG+LPS-induced THP-1 cells, and LINC00893, LINC00894, RUSC1-AS1, DBH-AS1, and RAMP2-AS1 were downregulated in the HG+LPS-induced THP-1 cells. Conclusions. lncRNAs and mRNAs are extensively linked and form a coexpression network, and lncRNAs may influence the development of type 2 diabetes by regulating the corresponding mRNAs. The ten key genes obtained may become biomarkers of inflammation in type 2 diabetes in the future.
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Academic Editor: Vijaya Anand
ISSN:1470-3203
1752-8976
1752-8976
DOI:10.1155/2023/6072438