Anti-adipogenic effect of Malva parviflora on 3T3-L1 adipocytes

• Published in: PloS one Vol. 19; no. 8; p. e0306903
• Main Authors: Méndez-Martínez, Marisol, Zamilpa, Alejandro, Zavala-Sánchez, Miguel A, Almanza-Pérez, Julio C, Jiménez-Ferrer, J Enrique, Herrera-Ruiz, Maribel, González-Cortázar, Manasés, Cervantes-Torres, Jaquelynne, Fragoso, Gladis, Rosas-Salgado, Gabriela
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 08.08.2024; Public Library of Science (PLoS)
• Subjects: 3T3-L1 Cells; Accumulation; Adipocytes; Adipocytes - cytology; Adipocytes - drug effects; Adipocytes - metabolism; Adipogenesis; Adipogenesis - drug effects; AKT protein; Alanine; Amino acids; Animals; Antihypertensives; Aprotinin; Biology and Life Sciences; Body fat; Cell culture; Cell Differentiation - drug effects; Chromatography; Differentiation; Ethylenediaminetetraacetic acid; Fatty acids; Fibroblasts; Insulin resistance; Interleukin 6; Interleukin-6 - metabolism; Interleukins; Intracellular; Kinases; Lipid Metabolism - drug effects; Lipids; Lipogenesis; Malva parviflora; Mass spectrometry; Medicine and Health Sciences; Metformin; Mice; NMR; Nuclear magnetic resonance; Obesity; Peroxisome proliferator-activated receptors; Phenylalanine; Physical Sciences; Phytochemicals; Plant Extracts - pharmacology; PPAR gamma - metabolism; Proline; Proto-Oncogene Proteins c-akt - metabolism; Scientific imaging; Solvents; Sucrose; Valine; Mexico
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0306903

Malva parviflora has shown anti-inflammatory, antihypertensive, antihyperlipidemic, and hypoglycemic effects. This study is aimed to evaluate the anti-adipogenic effect of M. parviflora on 3T3-L1 adipocytes. Fibroblast differentiation was induced either in the absence or presence of M. parviflora fractions (F3, F4, F7, F12, F13, F17, F18 and F19) for 4 days; F17 and 18 were the most effective fractions in reducing intracellular lipid accumulation (by 25.6% and 23.1%, respectively). EC50 of F17 and F18 (14 μg/mL and 17 μg/mL, respectively) were used to evaluate their anti adipogenic effect. After 10 days of inducing differentiation in the absence or presence of the extracts at the EC50 of F17 and F18, lipid accumulation, the concentration of interleukin 6 (IL-6) were measured in the culture medium; the presence of PPAR-γ, AKT, and p-AKT was also determined. In differentiated adipocytes (C2), F17 maintained intracellular lipid concentration at levels comparable to metformin, while decreasing PPAR-γ and increasing p-AKT presence; it also prevented IL-6 expression. F17 consists of alanine, valine, phenylalanine, and proline. On the other hand, F18 reduced intracellular lipid concentrations, prevented the increase of PPAR-γ and p-AKT, and maintained IL-6 expression at similar levels as metformin. F18 is mainly constituted by alanine, valine, proline, and sucrose. In conclusion, M. parviflora fractions (F17 and F18) control the process of adipogenesis, lipogenesis, and cellular dysfunction.