Chorioallantoic Membrane Assay at the Cross-Roads of Adipose-Tissue-Derived Stem Cell Research

• Published in: Cells (Basel, Switzerland) Vol. 12; no. 4; p. 592
• Main Authors: Oliinyk, Dmytro, Eigenberger, Andreas, Felthaus, Oliver, Haerteis, Silke, Prantl, Lukas
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 01.02.2023; MDPI
• Subjects: Adipose tissue; Adipose Tissue - metabolism; Adipose tissues; adipose-derived stem cells; Angiogenesis; Animals; Biochemical assays; Body fat; CAM assay; Chorioallantoic Membrane; Embryology; Experiments; Fetuses; Immunomodulation; Medical research; Membranes (Biology); Mesenchymal stem cells; Mesenchyme; Metabolism; Methods; Physiological aspects; Physiology; Precision medicine; Respiration; Review; Smooth muscle; Stem Cell Research; Stem cells; Tissue Engineering; tissue regeneration; Vascular endothelial growth factor; Wound healing; Germany; adipose-derived stem cells; tissue regeneration; chorioallantoic membrane; tissue engineering; wound healing; scaffolds; CAM assay; biocompatibility; mesenchymal stem cells
• ISSN: 2073-4409; 2073-4409
• DOI: 10.3390/cells12040592

With a history of more than 100 years of different applications in various scientific fields, the chicken chorioallantoic membrane (CAM) assay has proven itself to be an exceptional scientific model that meets the requirements of the replacement, reduction, and refinement principle (3R principle). As one of three extraembryonic avian membranes, the CAM is responsible for fetal respiration, metabolism, and protection. The model provides a unique constellation of immunological, vascular, and extracellular properties while being affordable and reliable at the same time. It can be utilized for research purposes in cancer biology, angiogenesis, virology, and toxicology and has recently been used for biochemistry, pharmaceutical research, and stem cell biology. Stem cells and, in particular, mesenchymal stem cells derived from adipose tissue (ADSCs) are emerging subjects for novel therapeutic strategies in the fields of tissue regeneration and personalized medicine. Because of their easy accessibility, differentiation profile, immunomodulatory properties, and cytokine repertoire, ADSCs have already been established for different preclinical applications in the files mentioned above. In this review, we aim to highlight and identify some of the cross-sections for the potential utilization of the CAM model for ADSC studies with a focus on wound healing and tissue engineering, as well as oncological research, e.g., sarcomas. Hereby, the focus lies on the combination of existing evidence and experience of such intersections with a potential utilization of the CAM model for further research on ADSCs.