Glucose biosensor based on immobilization of glucose oxidase in platinum nanoparticles/graphene/chitosan nanocomposite film

• Published in: Talanta (Oxford) Vol. 80; no. 1; pp. 403 - 406
• Main Authors: Wu, Hong, Wang, Jun, Kang, Xinhuang, Wang, Chongmin, Wang, Donghai, Liu, Jun, Aksay, Ilhan A., Lin, Yuehe
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 15.11.2009; Elsevier
• Subjects: Analytical chemistry; BASIC BIOLOGICAL SCIENCES; Biological and medical sciences; BIOLOGICAL INDICATORS; Biosensing Techniques - instrumentation; Biosensing Techniques - methods; Biosensor; Biosensors; Biotechnology; Chemistry; Chitosan - chemistry; COMPOSITE MATERIALS; Electrochemistry; Environmental Molecular Sciences Laboratory; Enzymes, Immobilized - chemistry; Enzymes, Immobilized - metabolism; Exact sciences and technology; Fundamental and applied biological sciences. Psychology; General, instrumentation; GLUCOSE; Glucose - analysis; Glucose - chemistry; Glucose Oxidase - chemistry; Glucose Oxidase - metabolism; Graphene; Graphite - chemistry; Hydrogen Peroxide - chemistry; Hydrogen Peroxide - metabolism; IMMOBILIZED CELLS; Methods. Procedures. Technologies; Microscopy, Electron, Transmission; Nanocomposite; Nanocomposites - chemistry; Nanocomposites - ultrastructure; Nanoparticles - chemistry; Nanoparticles - ultrastructure; NANOSTRUCTURES; OXIDASES; Oxidation-Reduction; PLATINUM; Platinum - chemistry; Platinum nanoparticles; Pt nanoparticle; SENSORS; Various methods and equipments; Nanocomposite; Platinum nanoparticles; Glucose; Graphene; Biosensor; Glucose oxidase; Ascorbic acid; Electrical conductivity; Hydrogen peroxide; Nanoparticle; Immobilization; Electrocatalysis; Signal; Platinum; Detection limit; Diagnosis; Stability; Enzyme; Uric acid; Chemical sensor; Reproducibility; Oxidoreductases; Chitosan; Surface area; Application
• ISSN: 0039-9140; 1873-3573
• DOI: 10.1016/j.talanta.2009.06.054

The bionanocomposite film consisting of glucose oxidase/Pt/functional graphene sheets/chitosan (GOD/Pt/FGS/chitosan) for glucose sensing is described. With the electrocatalytic synergy of FGS and Pt nanoparticles to hydrogen peroxide, a sensitive biosensor with a detection limit of 0.6 μM glucose was achieved. The biosensor also has good reproducibility, long-term stability and negligible interfering signals from ascorbic acid and uric acid comparing with the response to glucose. The large surface area and good electrical conductivity of graphene suggests that graphene is a potential candidate as a sensor material. The hybrid nanocomposite glucose sensor provides new opportunity for clinical diagnosis and point-of-care applications.