Desflurane impairs outcome of organotypic hippocampal slices in an in vitro model of traumatic brain injury
Decreased mortality and disability after traumatic brain injury is a significant medical challenge. Desflurane, a widely used volatile anesthetic has proven to be neuroprotective in a variety of in vitro and in vivo models of ischemic brain injury. The aim of this study was to investigate whether de...
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Published in | Medical gas research Vol. 6; no. 1; pp. 3 - 9 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
India
Wolters Kluwer - Medknow Publications
01.01.2016
Medknow Publications and Media Pvt. Ltd Medknow Publications & Media Pvt. Ltd Medknow Publications & Media Pvt Ltd |
Subjects | |
Online Access | Get full text |
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Summary: | Decreased mortality and disability after traumatic brain injury is a significant medical challenge. Desflurane, a widely used volatile anesthetic has proven to be neuroprotective in a variety of in vitro and in vivo models of ischemic brain injury. The aim of this study was to investigate whether desflurane exhibits neuroprotective properties in an in vitro model of traumatic brain injury. Organotypic hippocampal slice cultures were prepared from brains of 5-7-day-old C57/BL6 mouse pups. After 14 days of culture, the slices were subjected to a focal mechanical trauma and thereafter incubated with three different concentrations of desflurane (2, 4 and 6%) for 2, 24 and 72 hours. Cell injury was assessed with propodium iodide uptake. Our results showed that after 2 hours of desflurane exposure, no significant change in trauma intensity was observed. However, 2% and 4% desflurane could reduce the trauma intensity significantly in the no trauma group than in the no desflurane and trauma group. Incubation with 4% desflurane for 24 hours doubled the trauma intensity in comparison to the trauma control group and the trauma intensity further increased after 72 hours of incubation. Furthermore, a dose-dependent increase of trauma intensity after 24 hours exposure was observed. Our results suggest that a general neuroprotective attribute of desflurane in an in vitro model of traumatic brain injury was not observed. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Author contributions MK conducted the laboratory work, performed the statistical analysis and drafted the manuscript. AH, JL, LG helped to perform the study and draft the manuscript. RR participated in the study design and coordination and helped to draft the manuscript. MC conceived the study, participated in the study design and coordination and helped to draft the manuscript. All authors read and approved the final manuscript. |
ISSN: | 2045-9912 2045-9912 |
DOI: | 10.4103/2045-9912.179338 |