Identification of crystallin family proteins in vitreous body in rat endotoxin-induced uveitis: Involvement of crystallin truncation in uveitis pathogenesis

• Published in: Proteomics (Weinheim) Vol. 6; no. 11; pp. 3436 - 3444
• Main Authors: Bahk, Song-Chul, Lee, Sook-Hee, Jang, Jung-Un, Choi, Chang-Uk, Lee, Bok-Soo, Chae, Soo-Cheon, Song, Hye-Jin, Park, Zee-Yong, Yang, Yun-Sik, Chung, Hun-Taeg
• Format: Journal Article
• Language: English
• Published: Weinheim WILEY-VCH Verlag 01.06.2006; WILEY‐VCH Verlag; Wiley-VCH
• Subjects: Amino Acid Sequence; Analytical, structural and metabolic biochemistry; Animals; Biological and medical sciences; Crystallin; Crystallins - metabolism; Electrophoresis, Gel, Two-Dimensional; Endotoxin-induced uveitis; Fundamental and applied biological sciences. Psychology; Lipopolysaccharides; Male; Mass Spectrometry; Medical sciences; Miscellaneous; Molecular Sequence Data; Ophthalmology; Proteins; Proteome - metabolism; Rats; Rats, Inbred Lew; Truncation; Two-dimensional electrophoresis; Uvea diseases; Uveitis - chemically induced; Uveitis - metabolism; Vitreous Body - metabolism; Rat; Pathogenesis; Rodentia; Uvea disease; Identification; Endotoxin; Protein; Toxin; Eye disease; Vertebrata; Mammalia; Crystallin; otoxin-induced uveitis / Truncation / Two-dimensional electrophoresis; Proteomics; Uveitis
• ISSN: 1615-9853; 1615-9861
• DOI: 10.1002/pmic.200500779

Endotoxin‐induced uveitis (EIU) is an animal model of acute ocular inflammation. To characterize the mechanism of EIU, we analyzed the infiltration of proteins in the vitreous bodies of rats with EIU and normal rats using 2‐DE and micro LC/LC‐MS/MS. Twenty spots were identified in vitreous bodies of rats. Eighteen of these spots were members of the crystallin family. The truncated form of beta A4‐ and beta B2‐crystallin were predominant in normal vitreous bodies, but there were intact form of crystallins in lipopolysaccharide‐injected rats with EIU. These results suggest that crystallin family proteins are the major group of proteins involved in uveitic vitreous and that C‐terminal truncation of beta‐crystallins may play a role in EIU‐related disease progression.