Comparison of Different Irrigants in the Removal of Endotoxins and Cultivable Microorganisms from Infected Root Canals

• Published in: TheScientificWorld Vol. 2015; no. 2015; pp. 1 - 6
• Main Authors: Palo, Renato Miotto, Figueiredo, Mariana Diehl, Xavier, Ana Cláudia Carvalho, Chung, Adriana, Cardoso, Flávia Goulart da Rosa, Valera, Marcia Carneiro, Martinho, Frederico Canato
• Format: Journal Article
• Language: English
• Published: Cairo, Egypt Hindawi Publishing Corporation 2015; Hindawi Limited; Wiley
• Subjects: Antimicrobial agents; Bacteria; Bacterial Load; Bacteriology; Biomechanics; Candida albicans - drug effects; Cell division; Dental Pulp Cavity - microbiology; Dentin; Dentists; E coli; Endotoxins; Enterococcus faecalis - drug effects; Epoxy adhesives; Escherichia coli; Escherichia coli - drug effects; Gram-negative bacteria; Health aspects; Humans; Methods; Microbial Sensitivity Tests; Microorganisms; Pulpitis - drug therapy; Pulpitis - microbiology; Root Canal Irrigants - administration & dosage; Root canal therapy; Sodium; Surfactants; Teeth; Brazil
• ISSN: 2356-6140; 1537-744X; 1537-744X
• DOI: 10.1155/2015/125636

This study was conducted to compare the effectiveness of different irrigants used to remove endotoxins and cultivable microorganisms during endodontic therapy. Forty root canals were contaminated and divided into groups according to the irrigant: 2% NaOCl + surfactant, 2% CHX, 2.5% NaOCl, and pyrogen-free saline solution (control). Samples were collected after root canal contamination (S1), after instrumentation (S2), and 7 days after instrumentation (S3). Microorganisms and endotoxins were recovered from 100% of the contaminated root canals (S1). At S2, 2% NaOCl + surfactant, 2% CHX, and 2.5% NaOCl were able to completely eliminate cultivable microorganisms. At S3, both 2% CHX and 2.5% NaOCl were effective in preventing C. albicans and E. coli regrowth, but E. faecalis was still detected. No microorganism species was recovered from root canals instrumented with 2% NaOCl + surfactant. At S2, a higher percentage value of endotoxin reduction was found for 2% NaOCl + surfactant (99.3%) compared to 2% CHX (98.9%) and 2.5% NaOCl (97.18%) (p<0.05). Moreover, at S3, 2% NaOCl + surfactant (100%) was the most effective irrigant against endotoxins. All irrigants tested were effective in reducing microorganisms and endotoxins from root canals. Moreover, 2% NaOCl + surfactant was the most effective irrigant against endotoxins and regrowth of microorganisms.