Distribution and chemical coding of corticotropin-releasing factor-immunoreactive neurons in the guinea pig enteric nervous system
Immunofluorescence was used to study immunoreactivity (IR) for corticotropin‐releasing factor (CRF) in the guinea pig enteric nervous system. CRF‐IR was expressed in both the myenteric and the submucosal plexuses of all regions of the large and small intestine and the myenteric plexus of the stomach...
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Published in | Journal of comparative neurology (1911) Vol. 494; no. 1; pp. 63 - 74 |
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Main Authors | , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Hoboken
Wiley Subscription Services, Inc., A Wiley Company
01.01.2006
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Subjects | |
Online Access | Get full text |
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Summary: | Immunofluorescence was used to study immunoreactivity (IR) for corticotropin‐releasing factor (CRF) in the guinea pig enteric nervous system. CRF‐IR was expressed in both the myenteric and the submucosal plexuses of all regions of the large and small intestine and the myenteric plexus of the stomach. CRF‐IR nerve fibers were present in the myenteric and submucosal plexuses, in the circular muscle coat, and surrounding submucosal arterioles. Most of the CRF‐IR fibers persisted in the myenteric and submucosal plexuses after 7 days in organotypic culture. CRF‐IR was not coexpressed with tyrosine hydroxylase‐IR or calcitonin gene‐related peptide‐IR fibers. The proportions of CRF‐IR cell bodies in the myenteric plexus increased progressively from the stomach (0.6%) to the distal colon (2.8%). Most of the CRF‐IR myenteric neurons (95%) had uniaxonal morphology; the remainder had Dogiel type II multipolar morphology. CRF‐IR cell bodies in the myenteric plexus of the ileum expressed IR for choline acetyltransferase (56.9%), substance P (55.0%), and nitric oxide synthase (37.9%). CRF‐IR never colocalized with IR for calbindin, calretinin, neuropeptide Y, serotonin, or somatostatin in the myenteric plexus. CRF‐IR cell bodies were more abundant in the submucosal plexus (29.9–38.0%) than in the myenteric plexus. All CRF‐IR neurons in submucosal ganglia expressed vasoactive intestinal peptide‐IR and were likely to be secretomotor/vasodilator neurons. CRF‐IR neurons did not express IR for the CRF1 receptor. CRF1‐IR was expressed in neuronal neighbors of those with CRF‐IR. Collective evidence suggests that VIPergic secretomotor neurons might provide synaptic input to neighboring cholinergic neurons. J. Comp. Neurol. 494:63–74, 2006. © 2005 Wiley‐Liss, Inc. |
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Bibliography: | National Institutes of Health - No. R01 DK 37238; No. R01 DK 57075; No. KO8 DK 060468 istex:1E2A6960B48FAB32A1E6458AFDCBA044246B2F14 ark:/67375/WNG-JWTS6HXJ-7 ArticleID:CNE20781 Pharmaceutical Manufacturers of American Foundation ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Correspondence to: Jackie D. Wood, Ph.D. Department of Physiology and Cell Biology, College of Medicine, 304 Hamilton Hall, 1645 Neil Avenue, Columbus, OH 43210-1218, Tel. (614)292-5449, Fax (614)292-4888, E-mail wood.13@osu.edu |
ISSN: | 0021-9967 1096-9861 |
DOI: | 10.1002/cne.20781 |