Evaluation of higher plant virus resistance genes in the green alga, Chlorella variabilis NC64A, during the early phase of infection with Paramecium bursaria chlorella virus-1

Abstract With growing industrial interest in algae plus their critical roles in aquatic systems, the need to understand the effects of algal pathogens is increasing. We examined a model algal host–virus system, Chlorella variabilis NC64A and virus, PBCV-1. C. variabilis encodes 375 homologs to genes...

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Published inVirology (New York, N.Y.) Vol. 442; no. 2; pp. 101 - 113
Main Authors Rowe, Janet M, Dunigan, David D, Blanc, Guillaume, Gurnon, James R, Xia, Yuannan, Van Etten, James L
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 01.08.2013
Subjects
Chlorella
Chlorella - immunology
Chlorella - virology
Gene Expression Profiling
Gene Expression Regulation, Plant
Gene Expression Regulation, Viral
Host-Parasite Interactions
Infectious Disease
Paramecium bursaria
Phycodnaviridae
Phycodnaviridae - immunology
Phycodnaviridae - physiology
Phytoplankton
Post-transcriptional gene silencing
RNA directed DNA methylation
RNA Interference
RNA silencing
Transcriptome
Virus Replication
Phytoplankton
RNA silencing
Post-transcriptional gene silencing
RNA directed DNA methylation
Transcriptome
Phycodnaviridae
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Summary:Abstract With growing industrial interest in algae plus their critical roles in aquatic systems, the need to understand the effects of algal pathogens is increasing. We examined a model algal host–virus system, Chlorella variabilis NC64A and virus, PBCV-1. C. variabilis encodes 375 homologs to genes involved in RNA silencing and in response to virus infection in higher plants. Illumina RNA-Seq data showed that 325 of these homologs were expressed in healthy and early PBCV-1 infected (≤60 min) cells. For each of the RNA silencing genes to which homologs were found, mRNA transcripts were detected in healthy and infected cells. C. variabilis , like higher plants, may employ certain RNA silencing pathways to defend itself against virus infection. To our knowledge this is the first examination of RNA silencing genes in algae beyond core proteins, and the first analysis of their transcription during virus infection.
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Current Address: Department of Civil and Environmental Engineering, University of California, Irvine, CA 92697, United States.
ISSN:0042-6822
1096-0341
1096-0341
DOI:10.1016/j.virol.2013.04.018